{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T16:12:29Z","timestamp":1764432749812,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,11,2]],"date-time":"2010-11-02T00:00:00Z","timestamp":1288656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Introduction \u2013 Chiisanogenin existing in many<jats:italic> Acanthopanax<\/jats:italic> species has been reported to possess anti\u2010inflammatory, antibacterial and antiplatelet aggregatory activities.<\/jats:p><jats:p>Objective \u2013 To develop and validate a rapid and sensitive ultra performance liquid chromatography\u2010tandem mass spectrometry method for the determination of chiisanogenin in rat plasma and to investigate its pharmacokinetics after oral administration of chiisanogenin or the extract of <jats:italic>Acanthopanax sessiliflorus<\/jats:italic> fruits.<\/jats:p><jats:p>Methodology \u2013 The sample pretreatment involved a one\u2010step extraction of 0.2\u2009mL plasma with diethyl ether. Acetaminophen was used as the internal standard. The separation was carried out on an ACQUITY UPLC\u2122 BEH C<jats:sub>18<\/jats:sub> column with a mobile phase of acetonitrile\u20105\u2009mM ammonium acetate (90:10, <jats:italic>v\/v<\/jats:italic>) at a flow rate of 0.2\u2009mL\/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source.<\/jats:p><jats:p>Results \u2013 A high sample throughput was achieved with an analysis time of 1.1\u2009min per sample. The calibration curve was linear (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> \u2265 0.99) over the concentration range of 5\u2013500\u2009ng\/mL with a lower limit of quantification (LLOQ) of 5\u2009ng\/mL. The intra\u2010day and inter\u2010day precision (relative standard deviation, R.S.D.) values were below 11% and the accuracy (relative error, R.E.) was within 8% at all three quality control (QC) levels.<\/jats:p><jats:p>Conclusion \u2013 The method was successfully applied to the pharmacokinetic study of chiisanogenin in rat after oral administration of chiisanogenin and the extract of <jats:italic>Acanthopanax sessiliflorus<\/jats:italic> fruits. Other constituents in the extract affected the pharmacokinetic behavior of chiisanogenin. 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