{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:56:55Z","timestamp":1792223815569,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,4,4]],"date-time":"2011-04-04T00:00:00Z","timestamp":1301875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"crossref","award":["2009-0094074"],"award-info":[{"award-number":["2009-0094074"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Isoflavonoids from <jats:italic>Belamcanda chinensis<\/jats:italic> are known to have a number of physiological benefits including anti\u2010inflammatory, anti\u2010angiogenic and anti\u2010mutagenic properties. However, there have been no reports on the effective isolation and purification of isoflavonoids from <jats:italic>B<\/jats:italic>. <jats:italic>chinensis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop an efficient method for the preparative isolation and purification of isoflavones from <jats:italic>B<\/jats:italic>. <jats:italic>chinensis<\/jats:italic> by high\u2010speed counter\u2010current chromatography (HSCCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>A two\u2010step HSCCC isolation method was developed using solvent system of <jats:italic>n<\/jats:italic>\u2010hexane\u2013ethyl acetate\u20132\u2010propanol\u2013methanol\u2013water (5:6:2:3.5:6, v\/v) and of ethyl acetate\u2013methanol\u2013water (10:2:9, v\/v). FLASH purification system (45% methanol, isocratic) was also used for further purification. The purities and chemical structures of the isolated compounds were determined by high\u2010performance liquid chromatography\u2013photodiode array detection (HPLC\u2010PDA), electrospray ionisation\u2010mass spectrometry (ESI\u2010MS), <jats:sup>1<\/jats:sup>H\u2010 and <jats:sup>13<\/jats:sup>C\u2010nuclear magnetic resonance spectrometry (NMR) and nuclear overhauser enhancement (NOE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HSCCC was successfully used for the preparative separation and purification of seven isoflavones, including tectoridin (145.4\u2009mg, 97.5%), iridin (77.9\u2009mg, 94.0%), irilin D (42.0\u2009mg, 92.0%), tectorigenin (294.1\u2009mg, 98.6%), iristectorigenin A (86.8\u2009mg, 93.4%), irigenin (141.8\u2009mg, 95.8%) and irisflorentin (73.4\u2009mg, 94.7%) from the rhizomes of <jats:italic>B<\/jats:italic>. <jats:italic>chinensis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Two isoflavone glycosides and five isoflavone derivatives were successfully isolated and purified from the crude methanol extract of dried rhizomes of the <jats:italic>B<\/jats:italic>. <jats:italic>chinensis<\/jats:italic> by HSCCC. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.1306","type":"journal-article","created":{"date-parts":[[2011,4,4]],"date-time":"2011-04-04T07:20:31Z","timestamp":1301901631000},"page":"468-473","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Preparative Isolation and Purification of Seven Isoflavones from <i>Belamcanda chinensis<\/i>"],"prefix":"10.1002","volume":"22","author":[{"given":"Yeon Sil","family":"Lee","sequence":"first","affiliation":[{"name":"Center for Efficacy Assessment and Development of Functional Foods and Drugs Hallym University  Chuncheon 200\u2010702 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seon Ha","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Efficacy Assessment and Development of Functional Foods and Drugs Hallym University  Chuncheon 200\u2010702 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Kyu","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Natural Medicine Hallym University  Chuncheon 200\u2010702 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanghyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Applied Plant Science Chung\u2010Ang University  Anseong 456\u2010756 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Hoon","family":"Jung","sequence":"additional","affiliation":[{"name":"Natural Products Research Center Korea Institute of Science and Technology (KIST) Gangneung Institute  Gangneung 210\u2010340 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon Sung","family":"Lim","sequence":"additional","affiliation":[{"name":"Center for Efficacy Assessment and Development of Functional Foods and Drugs Hallym University  Chuncheon 200\u2010702 Republic of Korea"},{"name":"Institute of Natural Medicine Hallym University  Chuncheon 200\u2010702 Republic of Korea"},{"name":"Department of Food Science and Nutrition Hallym University  Chuncheon 200\u2010702 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,4,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(83)80045-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1248\/yakushi1947.93.12_1655"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/np000560b"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900632"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.02.091"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)91638-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/np980271b"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.49.1229"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.12.044"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-41125"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02980104"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.56.1452"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(99)00067-0"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900620"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.05.082"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826070903188245"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)97090-0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(92)80300-4"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(99)00621-4"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200700569"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.87.61"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2010.01.004"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-960976"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200500464"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/10286020008039908"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.1306","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.1306","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.1306","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T09:33:24Z","timestamp":1761039204000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.1306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4,4]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,9]]}},"alternative-id":["10.1002\/pca.1306"],"URL":"https:\/\/doi.org\/10.1002\/pca.1306","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"value":"0958-0344","type":"print"},{"value":"1099-1565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,4,4]]},"assertion":[{"value":"2010-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-10-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-04-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}