{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:49:11Z","timestamp":1790758151894,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,3,22]],"date-time":"2011-03-22T00:00:00Z","timestamp":1300752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013286","name":"Research Fund for the Doctoral Program of Higher Education of China","doi-asserted-by":"crossref","award":["20100096120004"],"award-info":[{"award-number":["20100096120004"]}],"id":[{"id":"10.13039\/501100013286","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The whole herb of <jats:italic>Potentilla discolor<\/jats:italic> has long been used for treatment of diarrhoea, malaria, haemoptysis and haematemesis in clinical applications. However, until now, there has been no literature regarding to the quality control of it.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop a simple and accurate HPLC\u2010DAD (diode array detection)\u2013ESI\/MS<jats:italic><jats:sup>n<\/jats:sup><\/jats:italic> (electrospray ionisation multistage mass spectrometry) method for the identification of constituents in <jats:italic>P. discolor<\/jats:italic> and simultaneous quantification of its marker compounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Separations were performed on a Shimpack C<jats:sub>18<\/jats:sub> column by gradient elution using acetonitrile:0.1% formic acid. The identification of constituents in <jats:italic>P. discolor<\/jats:italic> were achieved by HPLC\u2010DAD\u2010ESI\/MS<jats:italic><jats:sup>n<\/jats:sup><\/jats:italic> while six flavonoids and five triterpenoids were determined by HPLC\u2010DAD at 360 and 210\u2009nm, repectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 23 compounds including 13 flavonoids and 10 triterpenoids were identified or tentatively characterised. A quantitative HPLC\u2010DAD method allowing the simultaneously quantification of 11 marker compounds was optimised and validated for linearity, precision, accuracy, and limits of detection and quantification. The method was successfully applied to the quantification of 11 marker compounds in 10 samples of <jats:italic>P. discolor<\/jats:italic> collected from different provinces of China.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This developed method was validated as simple, precise and accurate, and could be used for effectively and comprehensibly evaluating the quality of <jats:italic>P. discolor<\/jats:italic>. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.1317","type":"journal-article","created":{"date-parts":[[2011,3,22]],"date-time":"2011-03-22T04:31:04Z","timestamp":1300768264000},"page":"547-554","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Quality Evaluation of <i>Potentilla discolor<\/i> by High\u2010performance Liquid Chromatography Coupled with Diode Array Detection and Electrospray Ionisation Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"22","author":[{"given":"Jie","family":"Yang","sequence":"first","affiliation":[{"name":"Key Laboratory of Modern Chinese Medicines China Pharmaceutical University  Nanjing 210009 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010dong","family":"Wen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Modern Chinese Medicines China Pharmaceutical University  Nanjing 210009 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei\u2010xi","family":"Jia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Modern Chinese Medicines China Pharmaceutical University  Nanjing 210009 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Mao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Modern Chinese Medicines China Pharmaceutical University  Nanjing 210009 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Modern Chinese Medicines China Pharmaceutical University  Nanjing 210009 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao\u2010xiang","family":"Lai","sequence":"additional","affiliation":[{"name":"Guangxi Academy of Chinese Medicine and Pharmaceutical Science  Guangxi 530022 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,3,22]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"981","article-title":"Protective effect of oleanolic acid and ursolic acid against lipid peroxidation","volume":"24","author":"Balanehru S","year":"1991","journal-title":"Biochem Int"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/S1044-0305(01)00226-4","article-title":"Determination of flavone, flavonol, and flavanone aglycones by negative ion liquid chromatography electrospray ion trap mass spectometry","volume":"12","author":"Fabre N","year":"2001","journal-title":"J Am Soc Mass Spectrom"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.794"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1218"},{"key":"e_1_2_5_6_1","first-page":"2705","volume-title":"Dictionary of Chinese Material Medica","author":"Jiangsu New Medical College","year":"1975"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.1134"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(95)90032-2"},{"key":"e_1_2_5_9_1","first-page":"588","article-title":"Effect of PDB on hyperglycemic animal models","volume":"20","author":"Meng LY","year":"2004","journal-title":"Chin Pharm Bull"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/74.4.418"},{"key":"e_1_2_5_11_1","first-page":"327","article-title":"Pentacyclic triterpenoic acids: new chemoprotective compounds. 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