{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:10:41Z","timestamp":1790863841892,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,6,21]],"date-time":"2011-06-21T00:00:00Z","timestamp":1308614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Dog's mercury (<jats:italic>Mercurialis perennis<\/jats:italic> L.) is a traditional European medicinal plant considered as a rich source of bioactive natural products. Yet phytochemical data of the plant are scant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to identify the hydrophilic phenolic constituents from <jats:italic>M. perennis<\/jats:italic> by aqueous and hydroalcoholic extraction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Extracts of herbal parts were investigated in\u2010depth by HPLC(DAD)\u2010MS\/MS and GC\/MS analyses. In addition, a novel compound was isolated and fully characterised by 1\u2010 and 2D\u2010NMR experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Several conjugates of caffeic, <jats:italic>p<\/jats:italic>\u2010coumaric and ferulic acids together with glucaric or 2\u2010hydroxyglutaric acids (depsides) were detected in the aqueous extracts from aerial plant parts by use of LC\u2010MS\/MS techniques as well UV\u2010spectral data. By implementation of preparative chromatography on polyamide pretreated with formic acid followed by vacuum liquid chromatography on reversed\u2010phase C<jats:sub>18<\/jats:sub>\u2010silica, one of the predominant depsides was isolated as a pure compound. The NMR spectra (<jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C NMR) together with 2D\u2010hetereonuclear multiple bond correlation NMR experiments (gHMBC and gHSQC) and chiral GC investigation, allowed identification of this compound as (\u2212)\u2010(<jats:italic>E<\/jats:italic>)\u2010caffeoyl\u20102\u2010(<jats:italic>R<\/jats:italic>)\u2010oxoglutarate. This structure was additionally supported by GC\/MS data after silylation and methylation reactions. The hydroalcoholic extract from aerial parts was separated by solvent partition between ethyl acetate and <jats:italic>n<\/jats:italic>\u2010butanol. The latter fraction (<jats:italic>n<\/jats:italic>\u2010butanol) yielded a mixture of mono\u2010 and oligo\u2010glycosides of kaempferol and quercetin, all of them being assigned by LC\u2010MS\/MS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present investigation constitutes the first comprehensive report on the hydrophilic constituents of the rarely studied plant <jats:italic>Mercurialis<\/jats:italic> and thus completes the phytochemical knowledge on <jats:italic>M. perennis<\/jats:italic>. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.1325","type":"journal-article","created":{"date-parts":[[2011,6,21]],"date-time":"2011-06-21T03:51:50Z","timestamp":1308628310000},"page":"60-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Investigations into the Phenolic Constituents of Dog's Mercury (<i>Mercurialis perennis<\/i> L.) by LC\u2010MS\/MS and GC\u2010MS analyses"],"prefix":"10.1002","volume":"23","author":[{"given":"Peter","family":"Lorenz","sequence":"first","affiliation":[{"name":"WALA Heilmittel GmbH, R&amp;D  Dorfstrasse 1, Bad Boll\/Eckwaelden 73087 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Conrad","sequence":"additional","affiliation":[{"name":"Hohenheim University Bioorganic Chemistry  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Bertrams","sequence":"additional","affiliation":[{"name":"WALA Heilmittel GmbH, R&amp;D  Dorfstrasse 1, Bad Boll\/Eckwaelden 73087 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Berger","sequence":"additional","affiliation":[{"name":"WALA Heilmittel GmbH, R&amp;D  Dorfstrasse 1, Bad Boll\/Eckwaelden 73087 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarina","family":"Duckstein","sequence":"additional","affiliation":[{"name":"WALA Heilmittel GmbH, R&amp;D  Dorfstrasse 1, Bad Boll\/Eckwaelden 73087 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Meyer","sequence":"additional","affiliation":[{"name":"WALA Heilmittel GmbH, R&amp;D  Dorfstrasse 1, Bad Boll\/Eckwaelden 73087 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian C.","family":"Stintzing","sequence":"additional","affiliation":[{"name":"WALA Heilmittel GmbH, R&amp;D  Dorfstrasse 1, Bad Boll\/Eckwaelden 73087 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,6,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.1053"},{"key":"e_1_2_6_3_1","first-page":"433","article-title":"Poisoning with Mercurialis perennis in a grazing cow herd. 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