{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:30:57Z","timestamp":1761093057619,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T00:00:00Z","timestamp":1319155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The Kang\u2010nao\u2010shuai (KNS) capsule is a combined herbal prescription used in the treatment of insomnia, amnesia, neurasthenia, age\u2010related dementia and brain injuries. Multiple constituents are considered to be responsible for the therapeutic effects of this herbal prescription. However, the quality control of the multicomponents is limited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To establish a liquid chromatography\u2013electrospray ionisation\u2013mass spectrometry method for the analysis of 40 constituents in KNS capsules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>The optimal chromatographic conditions were achieved on an Agilent C<jats:sub>18<\/jats:sub>\u2010column with a gradient elution that consisted of methanol and 0.1% formic acid in water. The precursor and product ions of analytes were monitored on a hybrid quadrupole linear ion trap mass spectrometer in positive and negative mode respectively using multiple\u2010reaction monitoring.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 40 constituents including organic acid, flavonoid, quinone, terpene, alkaloid and saponin were quantified, most of the 40 components were determined for the first time in the KNS capsule. A quantitative HPLC\u2013ESI\u2013MS\/MS method allowing the quantification of 40 marker compounds was optimised and validated for linearity, precision, accuracy, stability, specificity and limits of detection and quantification. The method was successfully applied to analyse 10 batches of KNS capsule.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The established method is simple and can be used as a tool for quality evaluation and control of this natural product. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.1366","type":"journal-article","created":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T12:29:33Z","timestamp":1319200173000},"page":"365-372","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Quality Evaluation of a Herbal Prescription Through Quantification of 40 Components by HPLC\u2013ESI\u2013MS\/MS"],"prefix":"10.1002","volume":"23","author":[{"given":"Man","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis, School of Pharmacy Hebei Medical University  361 East Zhongshan Road Shijiazhuang 050017 PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songchen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, School of Pharmacy Hebei Medical University  361 East Zhongshan Road Shijiazhuang 050017 PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowei","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, School of Pharmacy Hebei Medical University  361 East Zhongshan Road Shijiazhuang 050017 PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, School of Pharmacy Hebei Medical University  361 East Zhongshan Road Shijiazhuang 050017 PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingfeng Du\u2019 Lantong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, School of Pharmacy Hebei Medical University  361 East Zhongshan Road Shijiazhuang 050017 PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of 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