{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:45:46Z","timestamp":1791254746121,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,2,21]],"date-time":"2013-02-21T00:00:00Z","timestamp":1361404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>In the search for new natural compounds with acetylcholinesterase (AChE) inhibitory activity this study focused on galbanum, the oleo gum\u2010resin from <jats:italic>Ferula gummosa<\/jats:italic> Boiss., which had shown AChE inhibitory activity in a screening.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The isolation of bioactive compounds from plant extracts usually is laborious and time consuming. In an approach to accelerate the characterisation of compounds with AChE inhibitory activity, the potential of a combination of HPTLC bioautography with HPTLC\u2013MS\/NMR for the fast identification of active compounds in galbanum was studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Pre\u2010fractionation of the dichloromethane extract was performed by vacuum liquid chromatography. The resulting fractions were separated by HPTLC and active zones determined by bioautography. A TLC\u2013MS interface was used to elute the single zones from the plates directly into a mass spectrometer. The interface was also used to extract the two major active zones from HPTLC plates for off\u2010line one\u2010 and two\u2010dimensional NMR and quadrupole time of flight (QTOF) MS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The isolated compounds were identified as 7\u2010{[(2E)\u20103,7\u2010dimethylocta\u20102,6\u2010dien\u20101\u2010yl]oxy}\u20102H\u2010chromen\u20102\u2010one (auraptene) and 7\u2010{[(1R,4aR,6S,8aS)\u20106\u2010hydroxy\u20105,5,8a\u2010trimethyl\u20102\u2010methylenedecahydronaphthalen\u20101\u2010yl]methoxy}\u20102H\u2010chromen\u20102\u2010one (farnesiferol A). This is the first report of these substances in <jats:italic>F. gummosa<\/jats:italic>. Their median inhibitory concentration (IC<jats:sub>50<\/jats:sub>) values for AChE inhibition were determined as 47 and 17 \u00b5g\/mL in comparison with physostigmine as a positive control (IC<jats:sub>50<\/jats:sub>: 0.8 \u00b5g\/mL) and their concentrations in galbanum were quantified by HPLC as 3.5% and 7.9%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The study showed that HPTLC\u2013MS\/NMR can be considered as a fast and high\u2010confidence method for dereplication of natural compounds. From the correlation of the concentration of the elucidated compounds and their IC<jats:sub>50<\/jats:sub> values for AChE inhibition it can be concluded that auraptene and farnesiferol A are contributing to this activity of galbanum. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2422","type":"journal-article","created":{"date-parts":[[2013,2,21]],"date-time":"2013-02-21T12:25:18Z","timestamp":1361449518000},"page":"395-400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Combination of Bioautography with HPTLC\u2013MS\/NMR: A Fast Identification of Acetylcholinesterase Inhibitors from Galbanum<sup>\u2020<\/sup>"],"prefix":"10.1002","volume":"24","author":[{"given":"Hamid\u2010Reza","family":"Adhami","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uta","family":"Scherer","sequence":"additional","affiliation":[{"name":"Institute for Chemistry and Bioanalytics University of Applied Sciences Northwestern Switzerland  Muttenz 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