{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T23:56:44Z","timestamp":1772150204399,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T00:00:00Z","timestamp":1369872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Science and Technology Programs of Guangdong Province","award":["program number 2011B020310008"],"award-info":[{"award-number":["program number 2011B020310008"]}]},{"name":"Natural Science Funds of Guangdong Province","award":["program number S201101000368"],"award-info":[{"award-number":["program number S201101000368"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Camptothecin, a widely used natural anti\u2010cancer drug, is difficult to extract and purify effectively from plants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop new and highly efficient extraction and purification methods for analysis and production of camptothecin from leaves and fruits of <jats:italic>Camptotheca acuminata<\/jats:italic> and <jats:italic>Nothapodytes pittosporoides<\/jats:italic> roots.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Dried materials were loaded in empty columns with fivefold 60% ethanol for leaves or 70% ethanol for fruits of <jats:italic>C. acumnata<\/jats:italic>, and sixfold 70% ethanol for <jats:italic>N. pittosporoides<\/jats:italic> roots. The columns were eluted with the same solvents at room temperature. Eluent was collected as extraction solution. Extraction solution from leaves and fruits of <jats:italic>C. acuminata<\/jats:italic> was vacuum\u2010evaporated to remove ethanol, precipitated at pH\u20098.0 to remove alkaline insolubles and fractionated with chloroform at pH\u20093.0, which yields a crude product with 70% purity. Extraction solution from <jats:italic>N. pittosporoides<\/jats:italic> roots was concentrated to 1\/10 volume and precipitated at pH\u20093.0, which yields a crude product with 60% purity. All crude products were purified by crystallisation. All steps were monitored by HPLC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Camptothecin was extracted from the three plant materials at a 98% rate with 15\u2010 or 18\u2010fold solvent for content analysis, or at a 97% rate with five\u2010 or sixfold solvent for production. All crude products were purified to 98%. The overall recovery rates of camptothecin from plant materials to purified products reached 92% or higher.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The new procedures are simple and highly efficient, and have multiple advantages for quantitative analysis and large production of camptothecin from plants. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2441","type":"journal-article","created":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T13:13:03Z","timestamp":1369919583000},"page":"623-630","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["New and Highly Efficient Column Chromatographic Extraction and Simple Purification of Camptothecin from <i>Camptotheca acuminata<\/i> and <i>Nothapodytes pittosporoides<\/i>"],"prefix":"10.1002","volume":"24","author":[{"given":"Xue\u2010Hua","family":"Zeng","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences South China Normal University  Guangzhou China 510631"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue\u2010Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences South China Normal University  Guangzhou China 510631"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan\u2010Shan","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences South China Normal University  Guangzhou China 510631"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui\u2010Lin","family":"Hao","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences South China Normal University  Guangzhou China 510631"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences South China Normal University  Guangzhou China 510631"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He","family":"Ni","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences South China Normal University  Guangzhou China 510631"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han\u2010Bing","family":"Han","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences South China Normal University  Guangzhou China 510631"},{"name":"College of Chemistry and Life Sciences Guangdong University of Petrochemical Technology  139 Guandu Road 2 Maoming City Guangdong 525000 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai\u2010Hang","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences South China Normal University  Guangzhou China 510631"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,5,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-287-2_27"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.11.020"},{"key":"e_1_2_5_4_1","first-page":"10","article-title":"Study on the separation of natural camptothecin and 10\u2010hydroxycamptothecin by column chromatography","volume":"4","author":"Guo ZJ","year":"2005","journal-title":"Acta Chin Med Pharmacol"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)38654-4"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-35652"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0484780"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986706777585004"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2009.08.001"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cep.2012.03.008"},{"key":"e_1_2_5_11_1","first-page":"197","article-title":"Camptothecin distribution and content in Nothapodytes nimmoniana","volume":"6","author":"Mingzhang A","year":"2011","journal-title":"Nat Prod Commun"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2009.05.013"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1186219"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.06.009"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(01)00380-2"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(01)00345-0"},{"key":"e_1_2_5_17_1","first-page":"242","article-title":"Review of resources development and utilization of Nothapodytes sp. 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