{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:49:08Z","timestamp":1791251348847,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,6,24]],"date-time":"2013-06-24T00:00:00Z","timestamp":1372032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The tubers of <jats:italic>Pleione bulbocodioides<\/jats:italic> (Franch.) Rolfe, with gastrodin and benzyl ester glucosides as main components, have been used in traditional Chinese medicine for the treatment of various cancers and bacterial infections. Up to now, their official quality control method is still inadequate, and the difficulty of obtaining these high\u2010polarity compounds is one of the major reasons.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop a rapid and efficient method for preparative separation of the high\u2010polarity compounds gastrodin and benzyl ester glucosides.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An optimised solvent system composed of <jats:italic>n<\/jats:italic>\u2010butanol:ethanol:water (20:1:20, v\/v\/v) was applied for the elution\u2010extrusion counter\u2010current chromatography (EECCC) separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5\u2009mL\/min, a rotation speed of 850\u2009rpm and a temperature of 35\u00b0C.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five high\u2010polarity glucosides, including two new compounds, (<jats:italic>E<\/jats:italic>)\u20104\u2010<jats:italic>\u03b2<\/jats:italic>\u2010D\u2010glucopyranosyloxycinnamic acid 9\u2010(4\u2010<jats:italic>\u03b2<\/jats:italic>\u2010D\u2010glucopyranosyloxybenzyl) ester (4\u2009mg) and (<jats:italic>Z<\/jats:italic>)\u20102\u2010(2\u2010methylpropyl)butenedioic acid bis(4\u2010<jats:italic>\u03b2<\/jats:italic>\u2010D\u2010glucopyranosyloxybenzyl) ester (9\u2009mg), and three main components, gastrodin (87\u2009mg), dactylorhin A (60\u2009mg) and militarine (15\u2009mg), with HPLC purities of 95.4%, 96.4%, 91.1%, 97.2% and 95.5% respectively, were yielded from 400\u2009mg of the prepared sample.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Elution\u2010extrusion counter\u2010current chromatography could be used as a useful tool for the separation of high\u2010polarity compounds such as gastrodin and benzyl ester glucosides and the enrichment of the minor ones. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2453","type":"journal-article","created":{"date-parts":[[2013,6,24]],"date-time":"2013-06-24T22:56:39Z","timestamp":1372114599000},"page":"671-676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Elution\u2010extrusion Counter\u2010current Chromatography Separation of Two New Benzyl Ester Glucosides and Three Other High\u2010polarity Compounds from the Tubers of <i>Pleione bulbocodioides<\/i>"],"prefix":"10.1002","volume":"24","author":[{"given":"Yang","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy Shenyang Pharmaceutical University  Shenyang 110016 China"},{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 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