{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T05:23:58Z","timestamp":1770960238447,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T00:00:00Z","timestamp":1373328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Science and Technology Major Project \u2018Key New Drug Creation and Manufacturing Program\u2019, China","award":["Number: 2012ZX09301001-001; 2009ZX09301-001"],"award-info":[{"award-number":["Number: 2012ZX09301001-001; 2009ZX09301-001"]}]},{"name":"Key Program of the Science and Technology Commission of Shanghai Municipality, China","award":["Number: 09JC1416400"],"award-info":[{"award-number":["Number: 09JC1416400"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Cong\u2010Ming\u2010Tang (CMT), named smart\u2010soup in English, is a well\u2010known traditional Chinese medicine formula for the treatment of amnesia in China. However, the isolation, purification and identification procedures of the major bioactive constituents in CMT are difficult and time consuming.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To establish a rapid and sensitive high\u2010performance liquid chromatography\/electrospray ionisation with quadrupole time\u2010of\u2010flight tandem mass spectrometry (HPLC\u2013QTOF\/MS\/MS) method that could be applied to rapidly separate and identify the major bioactive constituents in CMT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Methanolic extract of CMT was used for HPLC\u2013QTOF\/MS\/MS analysis. Separation was performed on an Agilent Poroshell 120 EC\u2010 C18 column (2.7 \u00d7100 mm .i.d., 2.7 \u00b5m) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Both positive and negative ion modes were employed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This analytical tool allowed the identification of 55 compounds from CMT formulae by comparing their retention times and MS spectra with those of authentic compounds or literature data in both positive and negative ion modes, including 4 xanthone C\u2010glycosides, 4 sucrose esters, 11 oligosaccharide multi\u2010esters,15 triterpene saponins, 15 triterpene acids, 2 lignans and 4 phenylpropanoids. Onjisaponin MF was tentatively elucidated as a new triterpene saponin based on the summarised fragmentation rules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HPLC\u2013QTOF\/MS\/MS provides a new powerful approach to identify the major chemical constituents in CMT rapidly and accurately. This study proposed a series of potential bioactive components without preparative isolation from the crude extract of CMT and indicated that the HPLC\u2013QTOF\/MS\/MS method also can be a promising tool for the analysis of other traditional Chinese medicines. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2454","type":"journal-article","created":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T11:56:39Z","timestamp":1373370999000},"page":"677-688","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Analysis of Multiple Constituents in Cong\u2010Ming\u2010Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High\u2010performance Liquid Chromatography with Quadrupole Time\u2010of\u2010flight Mass Spectrometry"],"prefix":"10.1002","volume":"24","author":[{"given":"Yun","family":"Ling","sequence":"first","affiliation":[{"name":"Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhixiong","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Materia Medica Chinese 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