{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T19:53:12Z","timestamp":1788465192517,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T00:00:00Z","timestamp":1373328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Hyoscyamine and scopolamine, anti\u2010cholinergic agents widely used in medicine, are typically obtained from plants grown under natural conditions. Since field cultivation entails certain difficulties (changeable weather, pests, etc.), attempts have been made to develop a plant <jats:italic>in vitro<\/jats:italic> culture system as an alternative source for the production of these compounds. During experiments to locate the limiting steps in the biotechnological procedure, it is important to monitor not only the levels of the final products but also the changes in the concentration of their precursors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop a HPTLC method for the separation and quantitation of the main tropane alkaloids hyoscyamine and scopolamine, their respective direct precursors littorine and anisodamine, and cuscohygrine, a product of a parallel biosynthetic pathway that shares a common precursor (<jats:italic>N<\/jats:italic>\u2010methyl\u2010\u2206<jats:sup>1<\/jats:sup>\u2010pyrrolium cation) with tropane alkaloids.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using alkaloid extracts from <jats:italic>Atropa baetica<\/jats:italic> hairy roots, different TLC chromatographic systems and developing procedures were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Full separation of all compounds was obtained on HPTLC Si60 F<jats:sub>254<\/jats:sub> plates preconditioned with mobile phase vapours (chloroform:methanol:acetone:25% ammonia ratios of 75:15:10:1.8, v\/v\/v\/v). The chromatograms were developed twice (at distances of 4.0 and 3.0 cm) in a Camag twin trough chamber and visualised with Dragendorff's reagent. Densitometric detection (\u03bb\u2009=\u2009190 and 520 nm) was used for quantitative analyses of the different plant samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This method can be recommended for quantitation of hyoscyamine, scopolamine, anisodamine, littorine and cuscohygrine in different plant material (field grown vs. <jats:italic>in vitro<\/jats:italic> cultures). Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2455","type":"journal-article","created":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T12:05:03Z","timestamp":1373371503000},"page":"29-35","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Multi\u2010development\u2013HPTLC Method for Quantitation of Hyoscyamine, Scopolamine and their Biosynthetic Precursors in Selected Solanaceae Plants Grown in Natural Conditions and as <i>In Vitro<\/i> Cultures"],"prefix":"10.1002","volume":"25","author":[{"given":"Zbigniew","family":"Jaremicz","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy Medical University of Gdansk  al. gen. J. Hallera 107 80\u2010416 Gdansk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Luczkiewicz","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Medical University of Gdansk  al. gen. J. Hallera 107 80\u2010416 Gdansk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariusz","family":"Kisiel","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Medical University of Gdansk  al. gen. J. Hallera 107 80\u2010416 Gdansk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Z\u00e1rate","sequence":"additional","affiliation":[{"name":"Instituto Canario de Investigaci\u00f3n del C\u00e1ncer Hospital Univ. La Candelaria  38010 Tenerife Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabil El","family":"Jaber\u2010Vazdekis","sequence":"additional","affiliation":[{"name":"Instituto Canario de Investigaci\u00f3n del C\u00e1ncer Hospital Univ. La Candelaria  38010 Tenerife Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Migas","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Medical University of Gdansk  al. gen. J. Hallera 107 80\u2010416 Gdansk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,9]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"91","volume-title":"Chromatography of Alkaloids \u2013 Part A: Thin\u2010Layer Chromatography","author":"Baerheim\u2010Svendsen A","year":"1983"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.756"},{"key":"e_1_2_6_4_1","first-page":"717","volume-title":"Studies in Natural Products Chemistry","author":"Christen P","year":"2000"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)01387-0"},{"key":"e_1_2_6_6_1","first-page":"237","volume-title":"Medicinal Plant Biotechnology","author":"Drager B","year":"2007"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)00022-4"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1556\/JPC.18.2005.2.7"},{"issue":"4","key":"e_1_2_6_9_1","first-page":"439","article-title":"Tropane alkaloids as medicinally useful natural products and their synthetic derivatives as new drugs","volume":"60","author":"Grynkiewicz G","year":"2008","journal-title":"Pharmacol Rep"},{"key":"e_1_2_6_10_1","first-page":"69","volume-title":"Applied Thin\u2010layer Chromatography","author":"Hahn\u2010Deinstrop E","year":"2007"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/b001713m"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00269811"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1556\/JPC.17.2004.2.2"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2800030305"},{"key":"e_1_2_6_15_1","first-page":"685","volume-title":"Thin Layer Chromatography in Phytochemistry","author":"Mroczek T","year":"2008"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.12.034"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1962.tb08052.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002990050565"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(93)85105-Z"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.05.039"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1556\/JPC.22.2009.5.11"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00195896"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-8258.49373"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo025666l"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-008045382-8.00068-X"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOEC.0000006489.76006.cb"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-006-9085-8"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.2455","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.2455","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.2455","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T13:32:50Z","timestamp":1761053570000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.2455"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,9]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1002\/pca.2455"],"URL":"https:\/\/doi.org\/10.1002\/pca.2455","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"value":"0958-0344","type":"print"},{"value":"1099-1565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,9]]},"assertion":[{"value":"2012-03-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-05-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}