{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:09:56Z","timestamp":1790813396932,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,9,23]],"date-time":"2013-09-23T00:00:00Z","timestamp":1379894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Fuzilizhong Pills (FZLZ), a modified form of a famous traditional Chinese medicine (TCM) Lizhong Wan in <jats:italic>Treatise on Febrile Disease<\/jats:italic>s and consisting of <jats:italic>Panax ginseng<\/jats:italic> C.A.Mey. (Ren Shen), <jats:italic>Aconitum carmichaelii<\/jats:italic> Debx. (Fu Zi, Zhi), <jats:italic>Glycyrrhiza uralensis<\/jats:italic> Fisch., <jats:italic>Glycyrrhiza inflata<\/jats:italic> Bat. or <jats:italic>Glycyrrhiza glabra<\/jats:italic> L. (Gan Cao), <jats:italic>Atractylodes macrocephala<\/jats:italic> Koidz. (Bai Zhu) and <jats:italic>Zingiber offcinale<\/jats:italic> Rosc. (Gan Jiang), show strong clinical therapeutic effects for dyspnea and pulmonary oedema. However, the bioactive compounds are still unclear. In this study, FZLZ was analysed using a rapid detection method to identify its anti\u2010inflammatory and spasmolytic constituents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop a simple screening method to detect the anti\u2010inflammatory and spasmolytic constituents of FZLZ.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ultra\u2010performance liquid chromatography with quadrupole time\u2010of\u2010flight mass spectrometry combined with dual\u2010bioactive (NF\u2010\u03baB and \u03b2<jats:sub>2<\/jats:sub>\u2010adrenergic receptor) luciferase reporter assay systems was employed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two \u03b2<jats:sub>2<\/jats:sub>\u2010adrenergic receptor agonists (salsolinol and higeramine) and three terpenoidal analogues of NF\u2010\u03baB inhibitors such as ginsenosides derivatives, triperpenoids derivatives and diester\u2010diterpenoid aconitum alkaloid derivatives were characterised. Mesaconitine, flaconitine, ginsenosides Rb2, Rf, Rg2, F1 and Ro were considered to be new NF\u2010\u03baB inhibitors. Furthermore, IL\u20108 detection by enzyme linked immunosorbent assay confirmed the anti\u2010inflammatory effects of the potential NF\u2010\u03baB inhibitors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Compared with conventional fingerprints, activity\u2010integrated fingerprints that contain both chemical and bioactive details offer a more comprehensive understanding of the chemical composition of plant materials. This strategy clearly demonstrated that dual bioactivity\u2010integrated fingerprinting is a powerful tool for the improved screening and identification of potential dual\u2010target lead compounds in complex herbal medicines. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2474","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T20:31:58Z","timestamp":1381523518000},"page":"113-121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Identification of Nuclear Factor\u2010\u03baB Inhibitors and \u03b2<sub>2<\/sub> Adrenergic Receptor Agonists in Chinese Medicinal Preparation Fuzilizhong Pills Using UPLC with Quadrupole Time\u2010of\u2010flight MS"],"prefix":"10.1002","volume":"25","author":[{"given":"Linyi","family":"Dong","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), College of Pharmacy Tianjin Medical University  300070 Tianjin 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