{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:11:34Z","timestamp":1784171494176,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,9,23]],"date-time":"2013-09-23T00:00:00Z","timestamp":1379894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Biflavonoids are the primary constituents of <jats:italic>Selaginella doederleinii<\/jats:italic> Hieron, to which different bioactivities have been attributed, including anti\u2010cancer, anti\u2010inflammatory, anti\u2010oxidant, anti\u2010fungal and anti\u2010virus activity. However, effective methods for separation of these compounds are not currently available.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop a high performance and bioassay\u2010guided method for preparative isolation of biflavonoids from <jats:italic>S<\/jats:italic>. <jats:italic>doederleini<\/jats:italic> via high\u2010speed counter\u2010current chromatography (HSCCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The anti\u2010proliferation effects of four fractions (70% ethanol, petroleum ether, dichloromethane and acetic ether extracts) of <jats:italic>S<\/jats:italic>. <jats:italic>doederleinii<\/jats:italic> on five human cancer cells were monitored. The dichloromethane and acetic ether extracts showed good cytotoxicities to the studied cancer cell lines, guiding the subsequent separation. Two solvent systems composed of <jats:italic>n<\/jats:italic>\u2010hexane:ethyl acetate:methanol:water (1:2:1.5:1.5, v\/v) and <jats:italic>n<\/jats:italic>\u2010hexane:ethyl acetate:methanol:water (3:2:3:2, v\/v) were developed for separation of the active fractions, respectively. Identification of the biflavonoids was performed by EI\u2010MS<jats:italic><jats:sup>n<\/jats:sup><\/jats:italic>, <jats:sup>1<\/jats:sup>H\u2010 and <jats:sup>13<\/jats:sup>C\u2010NMR.'<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Under the optimised conditions, 12.6 mg amentoflavone (91.4%), 6.6 mg robustaflavone (90.4%), 7.5 mg 2'', 3''\u2010dihydro\u20103', 3'''\u2010biapigenin (98.2%) and 7.3 mg 3', 3'''\u2010binaringenin (90.3%) from acetic ether extract (500 mg) and 6.3 mg heveaflavone (93.5%) and 5.3 mg 7, 4', 7'', 4'''\u2010tetra\u2010<jats:italic>O<\/jats:italic>\u2010methyl\u2010amentoflavone (94.5%) from dichloromethane extract (200 mg) were obtained, respectively. The anti\u2010proliferation effects of the six biflavonoids on the five human cancer cells were further verified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The study provides methodological references for simultaneously preparative isolation of several bioactive biflavones from the herbal family of <jats:italic>Selaginella<\/jats:italic>. It is the first report discovering 2'', 3''\u2010dihydro\u20103', 3'''\u2010biapigenin and 3', 3'''\u2010binaringenin from this herb and describing their cytotoxicities to human cancer cell lines. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2478","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T17:56:24Z","timestamp":1381514184000},"page":"127-133","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Preparative Isolation of six Anti\u2010Tumour Biflavonoids from <i>Selaginella Doederleinii<\/i> Hieron by High\u2010Speed Counter\u2010Current Chromatography"],"prefix":"10.1002","volume":"25","author":[{"given":"Shaoguang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis, Faculty of Pharmacy Fujian Medical University  Fuzhou 350004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meifeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Faculty of Pharmacy Fujian Medical University  Fuzhou 350004 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