{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T04:14:44Z","timestamp":1778127284235,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T00:00:00Z","timestamp":1391558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2014,5]]},"abstract":"ABSTRACT<\/jats:title>Introduction<\/jats:title>Chromones and pyrones are the major secondary metabolites of Aloe barbadensis<\/jats:italic> Miller. As they are minor components of the plant, an efficient purification procedure for them is of great importance for promoting their pharmacological studies.<\/jats:p><\/jats:sec>Objective<\/jats:title>To develop efficient methods for one\u2010step separation and purification of two chromones (5\u2010((S<\/jats:italic>)\u20102\u2032\u2010oxo\u20104\u2032\u2010hydroxypentyl)\u20102\u2010hydroxymethylchromone (1) and 5\u2010((4E<\/jats:italic>)\u20102\u2032\u2010oxo\u2010pentenyl)\u20102\u2010hydroxymethylchromone (3)) and one pyrone (aloenin aglycone (2)) from A<\/jats:italic>. barbadensis<\/jats:italic> via reversed\u2010phase flash chromatography (RP\u2010FC) and high\u2010speed counter current chromatography (HSCCC).<\/jats:p><\/jats:sec>Methods<\/jats:title>The RP\u2010FC separation was performed using methanol:water (26:74, v\/v) as the mobile phase at a flow rate of 20\u2009mL\/min. A solvent system composed of dichloromethane:methanol:water (3:1.5:1, v\/v\/v) was used for the HSCCC separation, at a flow rate of 2.0\u2009mL\/min.<\/jats:p><\/jats:sec>Results<\/jats:title>A one\u2010step RP\u2010FC operation within 110\u2009min was successfully used for the purification of compounds 1 (27.9\u2009mg, 96.5%), 2 (32.4\u2009mg, 98.2%) and 3 (4.1\u2009mg, 99.0%) from 129\u2009mg of crude sample, and a one\u2010step HSCCC separation within 95\u2009min was successfully implemented for the purification of compounds 1 (31.1\u2009mg, 97.6%), 2 (35.8\u2009mg, 96.7%) and 3 (2.7\u2009mg, 98.1%) from 134\u2009mg of crude sample.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The developed procedures were efficient, with low cost and high yield, which would afford sufficient amounts of high\u2010purity compounds for chromatographic purposes and pharmacological activity screening. Copyright \u00a9 2014 John Wiley & Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2504","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T00:45:20Z","timestamp":1391561120000},"page":"282-288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["One\u2010step Separation and Purification of Two Chromones and One Pyrone from Aloe barbadensis<\/i> Miller: a Comparison Between Reversed\u2010phase Flash Chromatography and High\u2010speed Counter Current Chromatography"],"prefix":"10.1002","volume":"25","author":[{"given":"Jia\u2010Sheng","family":"Zhong","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Sun Yat\u2010sen University Guangzhou 510006 PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin\u2010Zhi","family":"Wan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Sun Yat\u2010sen University Guangzhou 510006 PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Jing","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Sun Yat\u2010sen University Guangzhou 510006 PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Fang","family":"Wu","sequence":"additional","affiliation":[{"name":"Analysis and Testing Center Chinese Academy of Tropical Agricultural Sciences Haikou 571101 PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Yong","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Sun Yat\u2010sen University Guangzhou 510006 PR 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