{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T18:37:40Z","timestamp":1768070260339,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T00:00:00Z","timestamp":1435017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["31470426"],"award-info":[{"award-number":["31470426"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["31300292"],"award-info":[{"award-number":["31300292"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Open Topic of Key Laboratory of Tibetan Medicine Research, CAS","award":["2014-TMR-03"],"award-info":[{"award-number":["2014-TMR-03"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2015,11]]},"abstract":"Abstract<\/jats:title>\n \n Introduction<\/jats:title>\n \n The dried seeds of\n Iris lactea<\/jats:italic>\n have been used in traditional Chinese medicine. Previous studies have been focused on irisquinones while other chemical components are rarely reported.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n To establish an efficient high\u2010speed counter\u2010current chromatography (HSCCC) separation method with continuous sample load (CSL) and double\u2010pump balancing (DPB) mode to isolate proanthocyanidins from\n I. lactea<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Firstly, an ethyl acetate extract of\n I. lactea<\/jats:italic>\n was pre\u2010fractionated by silica column chromatography for the enrichment of proanthocyanidins. Secondly, the enriched proanthocyanidins sample (EPS) was further fractionated by HSCCC with a two\u2010phase solvent system ethyl acetate:\n n<\/jats:italic>\n \u2010butanol:water (9:1:10,\n v<\/jats:italic>\n \/\n v<\/jats:italic>\n \/\n v<\/jats:italic>\n ) using DPB mode. The flow rate of the two phases was 2.2\u2009mL\/min, the revolution speed was 900\u2009rpm, the separation temperature was 30\u2009\u00b0C and the detection wavelength was 280\u2009nm. Finally, the structures of the three isolated proanthocyanidins were elucidated by spectroscopic methods and compared with published data.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Under the optimized conditions, 600\u2009mg of the EPS with six continuous injections (100\u2009mg\/time) was fractionated, yielding 57\u2009mg of prodelphinidin B3, 198\u2009mg of procyanidin B3, and 162\u2009mg of procyanidin B1, at purities of 97.2%, 98.1% and 97.3%, respectively.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n The HSCCC separation method with CSL and DPB proved to be rapid, convenient and economical, constituting an efficient strategy for the isolation of proanthocyanidins. Copyright \u00a9 2015 John Wiley & Sons, Ltd.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/pca.2579","type":"journal-article","created":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T07:42:49Z","timestamp":1435045369000},"page":"444-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Rapid separation of three proanthocyanidin dimers from\n Iris lactea<\/i>\n Pall.\n var<\/i>\n .\n Chinensi<\/i>\n s (Fisch.) Koidz by high\u2010speed counter\u2010current chromatography with continuous sample load and double\u2010pump balancing mode"],"prefix":"10.1002","volume":"26","author":[{"given":"Huanhuan","family":"Lv","sequence":"first","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China"},{"name":"University of Chinese Academy of Sciences Beijing 100049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenzhen","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China"},{"name":"University of Chinese Academy of Sciences Beijing 100049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenhua","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yourui","family":"Suo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honglun","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,23]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2006.08.014"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(03)00104-3"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2013.08.019"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf501312a"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2006.05.049"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.02.117"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2005.10.008"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.11.037"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.03.018"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.10.006"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.11.028"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2013.06.049"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2010.09.033"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2004.04.010"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(02)00936-5"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.12.025"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2008.04.031"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0300184"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201300653"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)82297-9"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.06.035"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1278"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201101073"},{"key":"e_1_2_5_25_1","first-page":"156","article-title":"Chemical studies on Iris pallasii Fisch. var. chinensis Fisch","volume":"38","author":"Wu SJ","year":"1980","journal-title":"Acta Chim Sinica"},{"key":"e_1_2_5_26_1","first-page":"767","article-title":"Chemical studies on Iris pallasii Fisch. var. chinensis Fisch. 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