{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:17:56Z","timestamp":1792120676414,"version":"4.4.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2017,5]]},"abstract":"Abstract<\/jats:title>\n \n Introduction<\/jats:title>\n \n Geigeria alata<\/jats:italic>\n is a traditional plant used in Sudanese folk medicine for treatment of diabetes, cough, epilepsy and intestinal complaints.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n To analyze phenolic acids in\n Geigeria alata<\/jats:italic>\n roots and leaves and to evaluate their antioxidant and antimicrobial activities.\n <\/jats:p>\n <\/jats:sec>\n \n Methodology<\/jats:title>\n Phenolic acids in the aqueous\u2010methanol extracts were identified by LC\u2013MS. Major compounds were isolated using low\u2010pressure liquid chromatography. The quantitative analysis of phenolic acids was performed by a validated HPLC\u2010UV method with limits of detection ranging from 0.04 to 0.57\u00a0\u03bcg\/mL. 2,2\u2010Diphenyl\u20101\u2010picrylhydrazyl (DPPH), 2,2\u2032\u2010azinobis\u2010(3\u2010ethylbenzothiazine\u20106\u2010sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) methods were used for antioxidant activity evaluation. In addition, the minimal inhibitory concentration and the minimal bactericidal concentration against a panel of pathogenic bacteria and fungi were determined by the broth microdilution test.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n For the first time protocatechuic, caffeic,\n p\u2010<\/jats:italic>\n coumaroylquinic, caffeoylsinapoylquinic, caffeoylferuloylquinic, three feruloylquinic, six caffeoylquinic acids, and a caffeic acid hexoside were detected in\n Geigeria alata<\/jats:italic>\n roots by LC\u2013MS. HPLC\u2010UV analyses showed that 3,5\u2010dicaffeoylquinic acid (25.96\u00a0\u00b1\u00a02.08\u00a0mg\/g dry weight (DW)) was the most abundant phenolic acid in roots, while 4,5\u2010dicaffeoylquinic acid (8.99\u00a0\u00b1\u00a00.56\u00a0mg\/g DW) was the main compound present in leaves. 3,5\u2010Dicaffeoylquinic acid demonstrated stronger radical scavenging activity and reducing power compared with the crude extracts and the positive control 5\u2010caffeoylquinic acid. 3,4,5\u2010Tricaffeoylquinic acid revealed the highest antibacterial potential against the penicillin sensitive and resistant\n \n Staphylococcus aureus<\/jats:italic>\n <\/jats:styled-content>\n strains\n ,<\/jats:italic>\n as well as methicillin\u2010resistant\n \n S. aureus<\/jats:italic>\n <\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The caffeoylquinic acids content of up to 6.22% in\n Geigeria alata<\/jats:italic>\n roots establishes this species as a new source rich in these bioactive molecules. Copyright \u00a9 2016 John Wiley & Sons, Ltd.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/pca.2658","type":"journal-article","created":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T22:33:07Z","timestamp":1480631587000},"page":"176-184","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["HPLC\u2010UV and LC\u2013MS Analyses of Acylquinic Acids in\n Geigeria alata<\/i>\n (DC) Oliv. & Hiern. and their Contribution to Antioxidant and Antimicrobial Capacity"],"prefix":"10.1002","volume":"28","author":[{"given":"Dimitrina","family":"Zheleva\u2010Dimitrova","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Medical University of Sofia 2, Dunav str. 1000 Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reneta","family":"Gevrenova","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Medical University of Sofia 2, Dunav str. 1000 Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya M.","family":"Zaharieva","sequence":"additional","affiliation":[{"name":"Department of Infectious Microbiology, The Stephan Angeloff Institute of Microbiology Bulgarian Academy of Sciences 26 Akad. G. Bonchev Str. 1113 Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hristo","family":"Najdenski","sequence":"additional","affiliation":[{"name":"Department of Infectious Microbiology, The Stephan Angeloff Institute of Microbiology Bulgarian Academy of Sciences 26 Akad. G. Bonchev Str. 1113 Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silviya","family":"Ruseva","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry Medical University of Sofia 2 Zdrave str. Sofia 1431 Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Lozanov","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry Medical University of Sofia 2 Zdrave str. Sofia 1431 Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vessela","family":"Balabanova","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Medical University of Sofia 2, Dunav str. 1000 Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakina","family":"Yagi","sequence":"additional","affiliation":[{"name":"Botany Department, Faculty of Science University of Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgi","family":"Momekov","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, pharmacotherapy and toxicology, Faculty of Pharmacy Medical University of Sofia 2, Dunav Str. 1000 Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanio","family":"Mitev","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry Medical University of Sofia 2 Zdrave str. Sofia 1431 Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"721","article-title":"A new validated HPTLC method for quantitative determination of 1,5\u2010dicaffeoylquinic acid in Inula crithmoides roots","volume":"25","author":"Aboul\u2010Ela MA","year":"2012","journal-title":"Pak J Pharm Sci"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf026187q"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf050046h"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf062314x"},{"key":"e_1_2_5_6_1","unstructured":"Clinical and Laboratory Standards Institute (CLSI).2009.Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Approved Standard. Third edition. M31\u2010A3.28. 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