{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T22:47:31Z","timestamp":1781909251305,"version":"3.54.5"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T00:00:00Z","timestamp":1484524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Annonaceous acetogenins (AAGs) constitute a group of environmental neurotoxins, possibly implicated in sporadic atypical Parkinsonism\/dementia complexes. The recent evidencing of complex mixtures of AAGs in edible fruits and derived food products requires efficient and practical analytical tools for an estimation of human exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop a simple method for the direct quantitation of the majority of AAGs (sub\u2010types 1a and 1b) within crude extracts, using commonly available <jats:sup>1<\/jats:sup>H\u2013NMR spectrometers, for food control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Method development was carried out on 400\u00a0MHz and 300\u00a0MHz spectrometers, for routine application on fruits crude extracts of <jats:italic>Annona muricata<\/jats:italic> L. The method was validated with annonacin and squamocin as reference compounds. Two internal standards (ISs), fumaric acid and dimethyl fumarate, were successfully used, in deuterated methanol (CD<jats:sub>3<\/jats:sub>OD) and deuterated chloroform (CDCl<jats:sub>3<\/jats:sub>), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Quantitation was carried out using signals corresponding to the deshielded ethylenic protons characterising most AAGs, at \u03b4 7.18 or \u03b4 6.98\u00a0ppm in CDCl<jats:sub>3<\/jats:sub>. The limit of quantification (LOQ) was 2.5\u00a0mM, with acceptable accuracy, and the limit of detection (LOD) was 0.5\u00a0mM. The AAGs contents measured in seven distinct fruit samples of <jats:italic>Annona muricata<\/jats:italic> ranged from 14\u00a0\u03bcmol to 226\u00a0\u03bcmol of AAGs per 100\u00a0g fresh pulp (i.e. 0.14\u00a0mmol to 1.3\u00a0mmol of AAGs per fruit).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A simple, accurate and specific method for quantification of AAGs content was developed and validated for routine application to fruit pulp crude extracts. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2668","type":"journal-article","created":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T11:00:15Z","timestamp":1484564415000},"page":"251-256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<sup>1<\/sup>H qNMR Quantification of Annonaceous Acetogenins in Crude Extracts of <i>Annona muricata<\/i> L. Fruit Pulp"],"prefix":"10.1002","volume":"28","author":[{"given":"Natacha","family":"Bonneau","sequence":"first","affiliation":[{"name":"Laboratoire de Pharmacognosie, BioCIS, Univ. Paris\u2010Sud, CNRS Universit\u00e9 Paris\u2010Saclay, UFR Pharmacie  Ch\u00e2tenay\u2010Malabry France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timoth\u00e9","family":"Cynober","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacognosie, BioCIS, Univ. Paris\u2010Sud, CNRS Universit\u00e9 Paris\u2010Saclay, UFR Pharmacie  Ch\u00e2tenay\u2010Malabry France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Christophe","family":"Jullian","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacognosie, BioCIS, Univ. 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Paris\u2010Sud, CNRS Universit\u00e9 Paris\u2010Saclay, UFR Pharmacie  Ch\u00e2tenay\u2010Malabry France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.20104"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/B500186M"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2012.02.007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"PH25","DOI":"10.1055\/s-0032-1320684","article-title":"Annonaceous acetogenins as environmental neurotoxins: Human exposure from edible Annona fruits","volume":"78","author":"Bonneau N","year":"2012","journal-title":"Planta Med"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.12264"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-6551-5_2"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.20632"},{"key":"e_1_2_6_9_1","first-page":"1","volume-title":"Technical Report 1\/2014. 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