{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T20:21:35Z","timestamp":1789244495937,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,1,26]],"date-time":"2017-01-26T00:00:00Z","timestamp":1485388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Catha edulis<\/jats:italic><\/jats:styled-content> (qat, khat, mirra) is a woody plant species that is grown and consumed in East Africa and Yemen for its stimulant alkaloids cathinone, cathine and norephedrine. Two Celastraceae species, in addition to qat, have been noted for their stimulant properties in ethnobotanical literature. Recent phylogenetic reconstructions place four genera in a clade sister to <jats:styled-content style=\"fixed-case\"><jats:italic>Catha edulis<\/jats:italic><\/jats:styled-content>, and these genera are primary candidates to search for cathine and related alkaloids.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>Determine if cathine or related alkaloids are present in species of Celastraceae other than <jats:styled-content style=\"fixed-case\"><jats:italic>Catha edulis<\/jats:italic><\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Leaf samples from 43 Celastraceae species were extracted in water followed by basification of the aqueous extract and partitioning with methyl\u2010<jats:italic>t<\/jats:italic>\u2010butyl ether to provide an alkaloid\u2010enriched fraction. The extract was derivatised in a two\u2010stage process and analysed using GC\u2013MS for the presence of cathine. Related alkaloids and other metabolites in this alkaloid\u2010enriched fraction were tentatively identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cathinone, cathine and norephedrine were not detected in any of the 43 Celastraceae species assayed other than <jats:styled-content style=\"fixed-case\"><jats:italic>Catha edulis<\/jats:italic><\/jats:styled-content>. However, the phenylalanine\u2010 or tyrosine\u2010derived alkaloid phenylethylamine was identified in five species. Nine species were found to be enriched for numerous sterol\u2010 and terpene\u2010like compounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results indicate that cathine is unique to <jats:styled-content style=\"fixed-case\"><jats:italic>Catha edulis<\/jats:italic><\/jats:styled-content>, and not the compound responsible for the stimulant properties reported in related Celastraceae species. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2671","type":"journal-article","created":{"date-parts":[[2017,1,26]],"date-time":"2017-01-26T06:26:37Z","timestamp":1485411997000},"page":"257-266","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Employing Two\u2010stage Derivatisation and GC\u2013MS to Assay for Cathine and Related Stimulant Alkaloids across the Celastraceae"],"prefix":"10.1002","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8858-7720","authenticated-orcid":false,"given":"Luke R.","family":"Tembrock","sequence":"first","affiliation":[{"name":"Department of Biology Colorado State University  Fort Collins CO 80523 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey D.","family":"Broeckling","sequence":"additional","affiliation":[{"name":"Proteomics and Metabolomics Facility Colorado State University  Fort Collins CO 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