{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:51:37Z","timestamp":1786978297875,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T00:00:00Z","timestamp":1494288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011002","name":"NSFC-Yunnan Joint Fund","doi-asserted-by":"crossref","award":["U1202263"],"award-info":[{"award-number":["U1202263"]}],"id":[{"id":"10.13039\/501100011002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100014219","name":"National Science Fund for Distinguished Young Scholars","doi-asserted-by":"crossref","award":["31525005"],"award-info":[{"award-number":["31525005"]}],"id":[{"id":"10.13039\/501100014219","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the National Basic Research Program of China (973 Program)","award":["2013CB127505"],"award-info":[{"award-number":["2013CB127505"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2017,9]]},"abstract":"Abstract<\/jats:title>\n \n Introduction<\/jats:title>\n \n Glandular trichomes of plants are biochemical factories that could produce, store and secrete copious pharmaceutically important natural products. The Labiatae plant\n \n Leonurus japonicus<\/jats:italic>\n <\/jats:styled-content>\n is an important traditional Chinese medicine used to treat gynecological diseases, and has abundant peltate glandular trichomes (PGTs), in which the secondary metabolites accumulated are still unknown.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n To study the secondary metabolites specifically accumulated in the PGTs of\n \n L. japonicus<\/jats:italic>\n <\/jats:styled-content>\n and their biological activities, and provide a new way to pinpoint bioactive natural products from plants.\n <\/jats:p>\n <\/jats:sec>\n \n Methodology<\/jats:title>\n \n Morphology of the trichomes on\n \n L. japonicus<\/jats:italic>\n <\/jats:styled-content>\n were observed under a scanning electron microscope. The PGTs of\n \n L. japonicus<\/jats:italic>\n <\/jats:styled-content>\n were precisely collected using laser microdissection (LMD) and analysed for their secondary metabolites with ultra performance liquid chromatography\u2010tandem mass spectrometry (UPLC\u2010MS\/MS). Targeted compounds were isolated with classical phytochemical methods, and their structures were elucidated by spectroscopic analysis. Biological activities were evaluated by\n in vitro<\/jats:italic>\n assays.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Two labdane diterpenoids, leoheterin (1) and galeopsin (2), were localised in the PGTs of\n \n L. japonicus<\/jats:italic>\n <\/jats:styled-content>\n . Antithrombotic activity of 1 in anti\u2010platelet aggregation assay induced by arachidonic acid was observed. Both compounds showed potential anti\u2010inflammatory activity by inhibiting proinflammatory cytokine TNF\u2010\u03b1. In addition, anti\u2010proliferative effect of both compounds on several cancer cell lines was also detected.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Two bioactive labdane diterpenoids were localised in the PGTs of\n \n L. japonicus<\/jats:italic>\n <\/jats:styled-content>\n . The findings suggested that it might be an efficient approach to explore bioactive natural products from the glandular trichomes of medicinal plants with LMD\u2010UPLC\/MS\/MS. Copyright \u00a9 2017 John Wiley & Sons, Ltd.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/pca.2687","type":"journal-article","created":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T02:22:41Z","timestamp":1494296561000},"page":"404-409","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Localisation of Two Bioactive Labdane Diterpenoids in the Peltate Glandular Trichomes of\n \n Leonurus japonicus<\/i>\n <\/scp>\n by Laser Microdissection Coupled with UPLC\u2010MS\/MS"],"prefix":"10.1002","volume":"28","author":[{"given":"Chao\u2010Jiang","family":"Xiao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming 650201 P. R. China"},{"name":"Yunnan Key Laboratory of Natural Medicinal Chemistry Kunming 650201 P. R. China"},{"name":"University of Chinese Academy of Sciences Beijing 100049 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Chun","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming 650201 P. R. China"},{"name":"Yunnan Key Laboratory of Natural Medicinal Chemistry Kunming 650201 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi\u2010Hong","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming 650201 P. R. China"},{"name":"Yunnan Key Laboratory of Natural Medicinal Chemistry Kunming 650201 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Hua","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming 650201 P. R. China"},{"name":"Yunnan Key Laboratory of Natural Medicinal Chemistry Kunming 650201 P. R. China"},{"name":"University of Chinese Academy of Sciences Beijing 100049 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming 650201 P. R. China"},{"name":"Yunnan Key Laboratory of Natural Medicinal Chemistry Kunming 650201 P. R. 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