{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T15:48:43Z","timestamp":1792165723289,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T00:00:00Z","timestamp":1494460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>In order to conserve the biodiversity of <jats:italic>Capsicum<\/jats:italic> species and find genotypes with potential to be utilised commercially, Embrapa Clima Temperado maintains an active germplasm collection (AGC) that requires characterisation, enabling genotype selection and support for breeding programmes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of this study was to characterise pepper accessions from the Embrapa Clima Temperado AGC and differentiate species based on their metabolic profile using an untargeted metabolomics approach.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Cold (\u02d720\u00b0C) methanol extraction residue of freeze\u2010dried fruit samples was partitioned into water\/methanol (A) and chloroform (B) fractions. The polar fraction (A) was derivatised and both fractions (A and B) were analysed by gas chromatography coupled to mass spectrometry (GC\u2013MS). Data from each fraction was analysed using a multivariate principal component analysis (PCA) with XCMS software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Amino acids, sugars, organic acids, capsaicinoids, and hydrocarbons were identified. Outlying accessions including P116 (<jats:styled-content style=\"fixed-case\"><jats:italic>C. chinense<\/jats:italic><\/jats:styled-content>), P46, and P76 (<jats:styled-content style=\"fixed-case\"><jats:italic>C. annuum<\/jats:italic><\/jats:styled-content>) were observed in a PCA plot mainly due to their high sucrose and fructose contents. PCA also indicated a separation of P221 (<jats:styled-content style=\"fixed-case\"><jats:italic>C. annuum<\/jats:italic><\/jats:styled-content>) and P200 (<jats:styled-content style=\"fixed-case\"><jats:italic>C. chinense<\/jats:italic><\/jats:styled-content>), because of their high dihydrocapsaicin content.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although the metabolic profiling did not allow for grouping by species, it permitted the simultaneous identification and quantification of several compounds complementing and expanding the metabolic database of the studied <jats:italic>Capsicum<\/jats:italic> spp. in the AGC. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2692","type":"journal-article","created":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T23:02:15Z","timestamp":1494543735000},"page":"439-447","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Untargeted Metabolomic Analysis of <i>Capsicum<\/i> spp. by GC\u2013MS"],"prefix":"10.1002","volume":"28","author":[{"given":"Bianca Camargo","family":"Aranha","sequence":"first","affiliation":[{"name":"Graduate Programme in Food Science and Technology, Department of Agroindustrial Science and Technology, Faculty of Agronomy Eliseu Maciel Federal University of Pelotas  Caixa Postal 354, CEP 96010\u2010900 Pelotas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica Fernanda","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Graduate Programme in Food Science and Technology, Department 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