{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:27:23Z","timestamp":1761092843929,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T00:00:00Z","timestamp":1499817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The genus <jats:italic>Soldanella<\/jats:italic> is one of the few endemic to Europe. Some of its species have relevance in local traditional medicine. Earlier work has indicated the possible presence of saponins in <jats:styled-content style=\"fixed-case\"><jats:italic>S. alpina<\/jats:italic><\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate <jats:styled-content style=\"fixed-case\"><jats:italic>S. alpina<\/jats:italic><\/jats:styled-content> and other related species for the occurrence of saponins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Following sequential extraction with <jats:italic>n<\/jats:italic>\u2010hexane, dichloromethane and ethyl acetate the subsequent methanolic extract of <jats:styled-content style=\"fixed-case\"><jats:italic>S. alpina<\/jats:italic><\/jats:styled-content> roots was fractionated after solvent precipitation using fast centrifugal partition chromatography and column chromatography. Structures were elucidated by LC\u2010MS<jats:sup><jats:italic>n<\/jats:italic><\/jats:sup>, high\u2010resolution MS, hydrolysis experiments and one\u2010dimensional (1D)\u2010 and two\u2010dimensional (2D)\u2010NMR. A hydrophilic interaction liquid chromatography method was developed to quantitate saponins in the leaves and roots of four <jats:italic>Soldanella<\/jats:italic> species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Three triterpene saponins, two of them new natural products, were isolated from <jats:styled-content style=\"fixed-case\"><jats:italic>S. alpina<\/jats:italic><\/jats:styled-content>. Based on an epoxyoleanal aglycone substituted with four sugar units, they were analytically quantitated using a Kinetex 2.6 \u03bcm hydrophilic interaction liquid chromatography (HILIC) column together with a mobile phase comprising of ammonium acetate, water and acetonitrile. Method validation confirmed that the assay meets all requirements in respect to linearity, accuracy, sensitivity and precision. All four <jats:italic>Soldanella<\/jats:italic> species investigated contained the three saponins. The lowest total level of the three saponins (1.09%) was observed in <jats:styled-content style=\"fixed-case\"><jats:italic>S. montana<\/jats:italic><\/jats:styled-content> leaves while the highest saponin content (5.14%) was determined in <jats:styled-content style=\"fixed-case\"><jats:italic>S. alpina<\/jats:italic><\/jats:styled-content> roots.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The detection of saponins within the genus <jats:italic>Soldanella<\/jats:italic> is an indication that further phytochemical examination of this genus may reveal more secondary metabolites of interest. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2706","type":"journal-article","created":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T05:46:43Z","timestamp":1499838403000},"page":"567-574","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Isolation of Three Triterpene Saponins, Including Two New Oleanane Derivatives, from <i>Soldanella alpina<\/i> and Hydrophilic Interaction Liquid Chromatography\u2013Evaporative Light Scattering Detection of these Three Saponins in Four <i>Soldenella<\/i> Species"],"prefix":"10.1002","volume":"28","author":[{"given":"Julia","family":"Haller","sequence":"first","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Member of the CMBI University of Innsbruck  Innrain 80\u201082 6020 Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Schwaiger","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Member of the CMBI University of Innsbruck  Innrain 80\u201082 6020 Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"Stuppner","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Member of the CMBI University of Innsbruck  Innrain 80\u201082 6020 Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Gafner","sequence":"additional","affiliation":[{"name":"Mibelle Biochemistry  Bolimattstrasse 1 5033 Buchs Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7407-9060","authenticated-orcid":false,"given":"Markus","family":"Ganzera","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Member of the CMBI University of Innsbruck  Innrain 80\u201082 6020 Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2011.09.003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1271037"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2017.01.021"},{"key":"e_1_2_6_5_1","unstructured":"DudlerB StanglD.2015.Preparation to protect from extrinsic and intrinsic skin aging. 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