{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:33:38Z","timestamp":1791981218463,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T00:00:00Z","timestamp":1500940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Citrullus colocynthis<\/jats:italic><\/jats:styled-content> (L.) Schrad is extensively used to treat diabetes, obesity, fever, cancer, amenorrhea, jaundice, leukemia, rheumatism, and respiratory diseases. Chemical studies have indicated the presence of several cucurbitacins, flavones, and other polyphenols in this plant. These phytochemical constituents are responsible for the interesting antioxidant and other biological activities of <jats:styled-content style=\"fixed-case\"><jats:italic>C. colocynthis<\/jats:italic><\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>In the present study, for the first time, near infrared (NIR) spectroscopy coupled with partial least square (PLS) regression analysis was used to quantify the polyphenolic phytochemicals of <jats:styled-content style=\"fixed-case\"><jats:italic>C. colocynthis<\/jats:italic><\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>The fruit and aerial parts of the <jats:styled-content style=\"fixed-case\"><jats:italic>C. colocynthis<\/jats:italic><\/jats:styled-content> were extracted individually in methanol followed by fractionation in <jats:italic>n<\/jats:italic>\u2010hexane, chloroform, ethyl acetate, <jats:italic>n<\/jats:italic>\u2010butanol, and water. Near infrared (NIR) spectra were obtained in absorption mode in the wavelength range 700\u20132500\u00a0nm. The PLS regression model was then built from the obtained spectral data to quantify the total polyphenol contents in the selected plant samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The PLS regression model obtained had a <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> value of 99% with a 0.98 correlationship value and a good prediction with a root mean square error of prediction (RMSEP) value of 1.89% and correlation of 0.98. These results were further confirmed through UV\u2013vis spectroscopy and it is found that the ethyl acetate fraction has the maximum value for polyphenol contents (101.7\u00a0mg\/100\u00a0g; NIR, 100.4\u00a0mg\/100\u00a0g; UV\u2013vis).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The polyphenolic phytochemicals of the fruit and aerial parts of <jats:styled-content style=\"fixed-case\"><jats:italic>C. colocynthis<\/jats:italic><\/jats:styled-content> have been quantified successfully by using multivariate analysis in a non\u2010destructive, economical, precise, and highly sensitive method, which uses very simple sample preparation. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2710","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T07:02:14Z","timestamp":1500966134000},"page":"16-22","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Application of NIR Spectroscopy Coupled with PLS Regression for Quantification of Total Polyphenol Contents from the Fruit and Aerial Parts of <scp><i>Citrullus colocynthis<\/i><\/scp>"],"prefix":"10.1002","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4279-6399","authenticated-orcid":false,"given":"Tania S.","family":"Rizvi","sequence":"first","affiliation":[{"name":"UoN Chair of Oman's Medicinal Plants and Marine Natural Products University of Nizwa  Nizwa\u2010616 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Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2696-1163","authenticated-orcid":false,"given":"Javid","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Chemistry University of Nizwa  Nizwa\u2010616 Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farah","family":"Jabeen","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Malakand  KPK Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suryyia","family":"Manzoor","sequence":"additional","affiliation":[{"name":"Institute of Chemical Sciences Bahauddin Zakariya University  Multan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Al\u2010Harrasi","sequence":"additional","affiliation":[{"name":"UoN Chair of Oman's Medicinal Plants and Marine Natural Products University of Nizwa  Nizwa\u2010616 Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/81.1.317S"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf500080z"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2014.05.001"},{"key":"e_1_2_6_5_1","unstructured":"DukeJA.2007.Duke's Phytochemical and Ethnobotanical Databases Ethnobotanical uses ofCitrullus colocynthis(Cucurbitaceae).http:\/\/www.ars\u2010grin.gov\/cgi\u2010in\/duke\/ethnobot.pl[23 February 2017]."},{"key":"e_1_2_6_6_1","first-page":"1054","article-title":"A possible protective effect of \n                     Citrullus colocynthis\n                   Melon against diabetes mellitus type 2 associated with non\u2010alcoholic fatty liver syndrome in rats","volume":"8","author":"Esmail OEA","year":"2012","journal-title":"J Am 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