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A gel formulation of the drug has been approved by the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA) for the topical treatment of actinic keratosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop a rapid and reliable method for quantification of ingenol in various plant extracts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Methanolic extracts of 38 species of the <jats:italic>Euphorbia<\/jats:italic> genus were analysed via ultra\u2010high performance liquid chromatography with tandem mass spectrometry (UHPLC\u2013MS\/MS) after methanolysis and solid\u2010phase extraction (SPE) purification. The <jats:sup>18<\/jats:sup>O\u2013labelled ingenol analogue was prepared and used as an internal standard for ingenol content determination and method validation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The highest ingenol concentration (547\u00a0mg\/kg of dry weight) was found in the lower leafless stems of <jats:styled-content style=\"fixed-case\"><jats:italic>E. myrsinites<\/jats:italic><\/jats:styled-content>. The screening confirms a substantial amount of ingenol in species studied previously and furthermore, reveals some new promising candidates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The newly established UHPLC\u2013MS\/MS method shows to be an appropriate tool for screening of the <jats:italic>Euphorbia<\/jats:italic> genus for ingenol content and allows selection of species suitable for raw material production and\/or <jats:italic>in vitro<\/jats:italic> culture initiation. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2711","type":"journal-article","created":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T00:18:16Z","timestamp":1502151496000},"page":"23-29","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Quantitative Analysis of Ingenol in <i>Euphorbia<\/i> species via Validated Isotope Dilution Ultra\u2010high Performance Liquid Chromatography Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5845-6903","authenticated-orcid":false,"given":"Tibor","family":"B\u00e9res","sequence":"first","affiliation":[{"name":"Department of Chemical Biology and Genetics, Centre of the Region Han\u00e1 for Biotechnological and Agricultural Research Palack\u00fd University  \u0160lechtitel\u016f 27 CZ\u2010783 71 Olomouc Czech Republic"},{"name":"Central Laboratories and Research Support, Centre of the Region Han\u00e1 for Biotechnological and Agricultural Research Palack\u00fd University  \u0160lechtitel\u016f 27 CZ\u2010783 71 Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Dragull","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology and Genetics, Centre of the Region Han\u00e1 for Biotechnological and Agricultural Research Palack\u00fd University  \u0160lechtitel\u016f 27 CZ\u2010783 71 Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u0159\u00ed","family":"Posp\u00ed\u0161il","sequence":"additional","affiliation":[{"name":"Laboratory of Growth Regulators, Centre of the Region Han\u00e1 for Biotechnological and Agricultural Research Palack\u00fd University &amp; 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