{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T12:22:38Z","timestamp":1774959758315,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T00:00:00Z","timestamp":1504742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Oman Research Council (TRC)","award":["ORG\/HSS\/14\/004"],"award-info":[{"award-number":["ORG\/HSS\/14\/004"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>3\u2010O\u2010Acetyl\u201011\u2010keto\u2010<jats:italic>\u03b2<\/jats:italic>\u2010boswellic acid (AKBA), one of the pentacyclic triterpenoids, is the main biologically active constituent in the resin of <jats:styled-content style=\"fixed-case\"><jats:italic>Boswellia sacra<\/jats:italic><\/jats:styled-content> and has received significant pharmacological interest in recent years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>It was aimed to develop a robust method to quantify the AKBA content in methanolic extracts of different parts of <jats:styled-content style=\"fixed-case\"><jats:italic>B. sacra<\/jats:italic><\/jats:styled-content> plants and in various fractions of its resin exudates through near\u2010infrared spectroscopy (NIRS) coupled with partial least squares regression (PLSR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The near\u2010infrared (NIR) spectra were used to measure the AKBA standards and <jats:styled-content style=\"fixed-case\"><jats:italic>B. sacra<\/jats:italic><\/jats:styled-content> samples at a wavelength range between 700 and 2500\u00a0nm in absorption mode. A PLSR model was built from the obtained spectral data using 70% of the AKBA working standard solutions (training set), ranging from 0.1\u00a0ppm to 100\u00a0ppm. The final PLSR showed a <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> value of 99% with a root mean square error of cross\u2010validation (RMSECV) value of 0.39% and a <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> value of 99%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed that a 50% CHCl<jats:sub>3<\/jats:sub>\/<jats:italic>n<\/jats:italic>\u2010hexane sub\u2010fraction has the highest concentration of AKBA (14.8%), followed by 55% CHCl<jats:sub>3<\/jats:sub>\/<jats:italic>n<\/jats:italic>\u2010hexane (13.6%), and 40% CHCl<jats:sub>3<\/jats:sub>\/<jats:italic>n<\/jats:italic>\u2010hexane (6.1%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>As the results achieved with the proposed NIRS methodology are in close agreement to the results of AKBA analysis using HPLC, we suggest that our proposed NIRS method is a fast alternative and non\u2010destructive method for the analysis of AKBA in different samples of <jats:styled-content style=\"fixed-case\"><jats:italic>B. sacra<\/jats:italic><\/jats:styled-content>. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2721","type":"journal-article","created":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T16:09:41Z","timestamp":1504800581000},"page":"137-143","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Quantification of AKBA in <scp><i>Boswellia sacra<\/i><\/scp> Using NIRS Coupled with PLSR as an Alternative Method and Cross\u2010Validation by HPLC"],"prefix":"10.1002","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1563-225X","authenticated-orcid":false,"given":"Najeeb Ur","family":"Rehman","sequence":"first","affiliation":[{"name":"UoN Chair of Oman's Medicinal Plants and Marine Natural Products University of Nizwa  Nizwa \u2010616 Sultanate of 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