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This genus has unique medicinal properties. Consequently, appropriate methods need to be described for the identification of the chemical compounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>A liquid chromatography tandem mass spectrometry (LC\u2013MS\/MS) technique on a triple quadrupole mass spectrometer (TQMS) was used for separation and identification of leaf flavonoid compounds for seven <jats:italic>Phlomis<\/jats:italic> species including <jats:italic>Phlomis kurdia<\/jats:italic>, <jats:italic>Ph. aucheri<\/jats:italic>, <jats:italic>Ph. olivieri<\/jats:italic>, <jats:italic>Ph. bruguieri, Ph. persica, Ph. anisodonta<\/jats:italic> and <jats:italic>Ph. elliptica<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>The flavonoid solution of air\u2010dried leaves (10.5\u00a0g) was extracted using 85% methanol. The chromatogram was treated with three systems: methanol\u2013water, chloroform\u2013methanol and acetic acid. The extracts were analysed using LC\u2013MS\/MS. The MS<jats:sup>2<\/jats:sup> detection was performed under negative mode electrospray ionisation (ESI). The identification of constituents was based on authentic references used in the identification process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 35 chemical compounds were detected from which 32 were identified as flavonoids through comparison with published literature and reference standards. These compounds were distributed in four flavonoid classes. Flavones (12), flavonols (11), flavanones (8) and flavane (1) were the main groups appearing in almost all of the studied samples. The flavonoids such as naringenin, chrysoeriol, eriodictyol, dimethoxyflavanone, apigenin, luteolin, kaempferol and rhamnetin were in high proportions. Moreover, 22 flavonoid compounds were first reported in this study for <jats:italic>Phlomis<\/jats:italic> species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The fragmentation patterns of the compounds during collision induced dissociation (CID) clarified information of the compounds analysed. The detailed flavonoid compositions of <jats:italic>Phlomis<\/jats:italic> species provide the appropriate context from phytochemical and phytotherapeutics points of view. 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