{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:22:26Z","timestamp":1790011346118,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T00:00:00Z","timestamp":1524096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Marine sponge\u2010associated actinomycetes are potent sources of bioactive natural products of pharmaceutical significance. They also contributed to the discovery of several clinically relevant antimicrobials.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To apply the non\u2010targeted metabolomics approach in chemical profiling of the sponge\u2010derived bacterium <jats:italic>Rhodococcus<\/jats:italic> sp. UA13, formerly recovered from the Red Sea sponge <jats:italic>Callyspongia<\/jats:italic> aff. <jats:italic>Implexa<\/jats:italic>, along with testing for the anti\u2010infective potential of its different fractions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Metabolomic analysis of the crude extract was carried out using liquid chromatography with high resolution electrospray ionisation mass spectrometry (LC\u2010HR\u2010ESI\u2010MS) for dereplication purposes. Besides, the three major fractions (ethyl acetate, methanol, and <jats:italic>n<\/jats:italic>\u2010butanol) obtained by chromatographic fractionation of the crude extract were evaluated for their anti\u2010infective properties.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A variety of metabolites, mostly peptides, were characterised herein for the first time from the genus <jats:italic>Rhodococcus<\/jats:italic>. Among the tested samples, the <jats:italic>n<\/jats:italic>\u2010butanol fraction showed potent inhibitory activities against <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans<\/jats:italic><\/jats:styled-content>, and <jats:italic>Trypanosoma brucei brucei<\/jats:italic> with IC<jats:sub>50<\/jats:sub> values of 9.3, 6.7, and 8.7\u00a0\u03bcg\/mL, respectively, whereas only the ethyl acetate fraction was active against <jats:italic>Chlamydia trachomatis<\/jats:italic> (IC<jats:sub>50<\/jats:sub>\u00a0=\u00a018.9\u00a0\u03bcg\/mL). In contrast, both fractions did not exert anti\u2010infective actions against <jats:italic>Enterococcus faecalis<\/jats:italic> and <jats:italic>Leishmania major<\/jats:italic>, whereas the methanol fraction was totally inactive against all the tested organisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study showed the helpfulness of the established procedure in metabolic profiling of marine actinomycetes using liquid chromatography mass spectrometry (LC\u2013MS) data, which aids in reducing the complex isolation steps during their chemical characterisation. The anti\u2010infective spectrum of their metabolites is also interestingly relevant to future drug development.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2765","type":"journal-article","created":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T15:12:08Z","timestamp":1524150728000},"page":"543-548","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Metabolomic profiling and biological investigation of the marine sponge\u2010derived bacterium <i>Rhodococcus<\/i> sp. UA13"],"prefix":"10.1002","volume":"29","author":[{"given":"Yasmin","family":"Elsayed","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2425-0819","authenticated-orcid":false,"given":"John","family":"Refaat","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usama Ramadan","family":"Abdelmohsen","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Minia University  Minia Egypt"},{"name":"Department of Botany II, Julius\u2010von\u2010Sachs Institute for Biological Sciences University of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman Maher","family":"Othman","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Minia University  Minia Egypt"},{"name":"Department of Toxicology University of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helga","family":"Stopper","sequence":"additional","affiliation":[{"name":"Department of Toxicology University of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa Ahmed","family":"Fouad","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,19]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2012.06.005"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30323-1"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-802104-0.00030-5"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0138528"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2012.06.012"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3np70111e"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac202623g"},{"key":"e_1_2_4_9_1","first-page":"83","article-title":"The Genus Rhodococcus as a source of novel bioactive substances: A review","volume":"6","author":"Elsayed Y","year":"2017","journal-title":"J Pharmacogn 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