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Angiopteroside is a substance of pharmaceutical importance that is found in the rhizome of <jats:styled-content style=\"fixed-case\"><jats:italic>A. evecta<\/jats:italic><\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aims of this research are to improve the extraction method for angiopteroside from the rhizome, compared to that in a previous report, and to determine the yield of angiopteroside from the rhizome of <jats:styled-content style=\"fixed-case\"><jats:italic>A. evecta<\/jats:italic><\/jats:styled-content><jats:italic>,<\/jats:italic> as well as to obtain precise data for extractives from the rhizome of <jats:styled-content style=\"fixed-case\"><jats:italic>A. evecta<\/jats:italic><\/jats:styled-content> by using two\u2010dimensional NMR spectroscopy and liquid chromatography\u2013mass spectrometry (LC\u2013MS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>We investigated the chemical constituents of the whole rhizome by means of two\u2010dimensional NMR (heteronuclear single quantum coherence or HSQC) spectroscopy, neutral sugar analysis using the alditol acetate method, and lignin analysis using alkaline nitrobenzene oxidation and Klason lignin methods. LC\u2013MS revealed the purity of the angiopteroside. Antimicrobial assays were also performed for the purified angiopteroside by using a broth microdilution method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Angiopteroside was isolated by Soxhlet extraction with aqueous acetone followed by preparative thin\u2010layer chromatography (eluent: 20% methanol\/dichloromethane). LC\u2013MS revealed that angiopteroside can be found in the rhizome of <jats:styled-content style=\"fixed-case\"><jats:italic>A. evecta<\/jats:italic><\/jats:styled-content> in 9.9% yield, which is an extremely high yield for a plant extractive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HSQC analysis is a powerful tool for surveying compounds in plant materials, such as the whole rhizome of <jats:styled-content style=\"fixed-case\"><jats:italic>A. evecta<\/jats:italic><\/jats:styled-content>. Soxhlet extraction with aqueous acetone is an effective method for extracting glycosides from plant materials.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2794","type":"journal-article","created":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T20:29:21Z","timestamp":1537907361000},"page":"95-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Two\u2010dimensional NMR analysis of <scp><i>Angiopteris evecta<\/i><\/scp> rhizome and improved extraction method for angiopteroside"],"prefix":"10.1002","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0130-1919","authenticated-orcid":false,"given":"Hiroshi","family":"Kamitakahara","sequence":"first","affiliation":[{"name":"Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoki","family":"Okayama","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1804-4549","authenticated-orcid":false,"family":"Praptiwi","sequence":"additional","affiliation":[{"name":"Research Center for Biology Indonesian Institute of Sciences  Cibinong West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9226-6265","authenticated-orcid":false,"given":"Andria","family":"Agusta","sequence":"additional","affiliation":[{"name":"Research Center for Biology Indonesian Institute of Sciences  Cibinong West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7578-7392","authenticated-orcid":false,"given":"Yuki","family":"Tobimatsu","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8244-223X","authenticated-orcid":false,"given":"Toshiyuki","family":"Takano","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.03.111"},{"key":"e_1_2_5_3_1","series-title":"Plant Resources of South\u2010East Asia No 15(2)","first-page":"58","volume-title":"Cryptogams: Ferns and fern allies","author":"Winter WP","year":"2003"},{"key":"e_1_2_5_4_1","unstructured":"Garuda.Personal communication.2011."},{"key":"e_1_2_5_5_1","first-page":"187","article-title":"Inhibitory activities of angiopteroside for HIV\u20101 reverse transcriptase and lung cancer cell\u2010line","volume":"30","author":"Taveepanich S","year":"2005","journal-title":"J Sci Res Chulalongkorn Univ"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.11.037"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(01)97097-8"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.38.2862"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0567740881008091"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1139\/v68-122"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0490332"},{"key":"e_1_2_5_12_1","first-page":"603","article-title":"Aggressive chemicals in plants","author":"Reynolds T","year":"1975","journal-title":"Chem Ind\u2010London"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cber.19711040510"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf00062a013"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.064"},{"key":"e_1_2_5_16_1","volume-title":"Reference methods for dilution antimicrobial susceptibility testing for bacteria that grow aerobically. 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