{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T21:07:04Z","timestamp":1775941624342,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,6]],"date-time":"2018-11-06T00:00:00Z","timestamp":1541462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","award":["UGC\u2010UPE\u2010II program"],"award-info":[{"award-number":["UGC\u2010UPE\u2010II program"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Luffa acutangula<\/jats:italic><\/jats:styled-content> (L.) Roxb, commonly known as ridge gourd (cucurbitaceae), is a common vegetable cultivated in India. It is also a well\u2010used medicinal plant in Indian traditional medicine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To analyse the phenolics content of the most potent carbonic anhydrase\u2010inhibiting fraction from an extract of <jats:styled-content style=\"fixed-case\"><jats:italic>L. acutangula<\/jats:italic><\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>An aqueous ethanol extract of dried fruits of <jats:styled-content style=\"fixed-case\"><jats:italic>L. acutangula<\/jats:italic><\/jats:styled-content> was successively fractionated into petroleum ether, dichloromethane and ethyl acetate. The extract and subsequent fractions were assessed for carbonic anhydrase\u2010inhibitory activity and the enzyme inhibition kinetics were determined for the most active fraction. Total phenolic and flavonoid content of the extract and subsequent fractions were determined spectrophotometrically. Ultra\u2010performance liquid chromatography\u2010quadrupole\/time\u2010of\u2010flight mass spectrometry (UPLC\u2010QTOF\u2010MS) analysis was used to tentatively identify the major phenolics in the most active fraction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The concentration of total phenolics and total flavonoids in the extract and each fraction thereof correlated with the level of carbonic anhydrase inhibition activity. The ethyl acetate fraction of the aqueous ethanol extract of <jats:styled-content style=\"fixed-case\"><jats:italic>L. acutangula<\/jats:italic><\/jats:styled-content> had the highest carbonic anhydrase inhibition activity. The enzyme kinetics analysis indicated a mixed mode of inhibition. UPLC\u2010QTOF\u2010MS analysis of the ethyl acetate fraction indicated a number of phenolic acids, hydroxycoumarins, flavones, flavanones, and flavonoids.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The correlation of total phenolic content with carbonic anhydrase inhibition suggested further research that might confirm that phenolic compounds of <jats:styled-content style=\"fixed-case\"><jats:italic>L. acutangula<\/jats:italic><\/jats:styled-content> offer potential therapeutic benefits against carbonic anhydrase\u2010related disorders.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2800","type":"journal-article","created":{"date-parts":[[2018,11,6]],"date-time":"2018-11-06T21:39:53Z","timestamp":1541540393000},"page":"148-155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["UPLC\u2010QTOF\u2010MS analysis of a carbonic anhydrase\u2010inhibiting extract and fractions of <scp><i>Luffa acutangula<\/i><\/scp> (L.) Roxb (ridge gourd)"],"prefix":"10.1002","volume":"30","author":[{"given":"Joydeb","family":"Chanda","sequence":"first","affiliation":[{"name":"School of Natural Product Studies, Department of Pharmaceutical Technology Jadavpur University  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2859-3923","authenticated-orcid":false,"given":"Pulok K.","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"School of Natural Product Studies, Department of Pharmaceutical Technology Jadavpur University  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajarshi","family":"Biswas","sequence":"additional","affiliation":[{"name":"School of Natural Product Studies, Department of Pharmaceutical Technology Jadavpur University  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayan","family":"Biswas","sequence":"additional","affiliation":[{"name":"School of Natural Product Studies, Department of Pharmaceutical Technology Jadavpur University  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amrendra Kumar","family":"Tiwari","sequence":"additional","affiliation":[{"name":"School of Natural Product Studies, Department of Pharmaceutical Technology Jadavpur University  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashish","family":"Pargaonkar","sequence":"additional","affiliation":[{"name":"Agilent Technologies India Pvt. 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