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The main polyphenols present in the plant are quercetin (QCT), luteolin (LUT), 3\u2010<jats:italic>O<\/jats:italic>\u2010methylquercetin (3<jats:italic>O<\/jats:italic>MQ), and achyrobichalcone (ACB). However, the effective isolation and purification of these compounds from <jats:italic>A. satureioides<\/jats:italic> inflorescences are not an easy task.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop an efficient high\u2010performance counter\u2010current chromatography (HPCCC) method for quick separation and purification of naturally occurring phenolic compounds from the extract of <jats:italic>A. satureioides<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>A two\u2010step HPCCC semi\u2010preparative isolation method was developed using a solvent system composed of <jats:italic>n<\/jats:italic>\u2010hexane\/ethyl acetate\/methanol\/water (0.8:1.0:0.8:1.0) and dichloromethane\/methanol\/water (3.5:3.5:2.5).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The HPCCC method was used to obtain two fractions. The first fraction (F<jats:sub>1<\/jats:sub>) contained high levels of ACB, among other constituents, while the second fraction (F<jats:sub>2<\/jats:sub>) contained mostly QCT, LUT, and 3<jats:italic>O<\/jats:italic>MQ. Besides the high ACB content, F<jats:sub>1<\/jats:sub> contained three other flavonoid\u2010aglycones (kaempferol, 97.3%; isokaempferide, 92.4%; and 3,3\u2032\u2010di\u2010<jats:italic>O<\/jats:italic>\u2010methylquercetin, 95.2%) identified by an ultra\u2010performance liquid chromatography system coupled to a quadrupole time\u2010of\u2010flight with high\u2010definition mass spectrometry (UPLC\u2010QTOF\/HDMS) and nuclear magnetic resonance (NMR) analysis. Purity levels of ACB, 3<jats:italic>O<\/jats:italic>MQ, QCT, and LUT were 98.0, 97.0, 97.5, and 90.2%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first time that high purity ACB and six other flavonoids were obtained from <jats:italic>A. satureioides<\/jats:italic> inflorescences by HPCCC. These excellent results reveal the potential and versatility of HPCCC as a technique to produce different types of products from this plant species on a semi\u2010preparative scale: enriched fractions, new metabolites, or high purity compounds.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2803","type":"journal-article","created":{"date-parts":[[2018,12,19]],"date-time":"2018-12-19T08:27:07Z","timestamp":1545208027000},"page":"182-192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Semi\u2010preparative isolation and purification of phenolic compounds from <i>Achyrocline satureioides<\/i> (Lam) D.C. by high\u2010performance counter\u2010current chromatography"],"prefix":"10.1002","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3759-6587","authenticated-orcid":false,"given":"Sara Elis","family":"Bianchi","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em 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