{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T05:25:59Z","timestamp":1783661159201,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T00:00:00Z","timestamp":1543190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002648","name":"Catholic University of Korea","doi-asserted-by":"publisher","award":["2018"],"award-info":[{"award-number":["2018"]}],"id":[{"id":"10.13039\/501100002648","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102017R1A2B4003888"],"award-info":[{"award-number":["NRF\u20102017R1A2B4003888"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102018R1A6A1A03025108"],"award-info":[{"award-number":["NRF\u20102018R1A6A1A03025108"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Camellia japonica<\/jats:italic><\/jats:styled-content> L. (Theaceae) is an evergreen shrub, which is cultivated as a popular ornamental tree in Korea, China, and Japan and its seeds have been used as a source of cooking oil, in cosmetics and as a traditional medicine. Intensive phytochemical works have revealed that oleanane\u2010type saponins are the characteristic compounds of the seeds of <jats:italic><jats:styled-content style=\"fixed-case\">C. japonica<\/jats:styled-content><\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The purpose of the present study is to isolate and determine oleanane\u2010type saponins from <jats:styled-content style=\"fixed-case\"><jats:italic>C. japonica<\/jats:italic><\/jats:styled-content> using high\u2010performance countercurrent chromatography (HPCCC) coupled with reversed\u2010phase high\u2010performance liquid chromatography (RP\u2010HPLC) and spectroscopic evidences, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>HPLC electrospray ionisation quadrupole time\u2010of\u2010flight mass spectrometry (ESI\u2010Q\u2010TOF\u2010MS) was applied to profile the saponin composition of an enriched saponin extract of <jats:styled-content style=\"fixed-case\"><jats:italic>C. japonica<\/jats:italic><\/jats:styled-content> seeds. The enriched saponin extract was separated by HPCCC using a dichloromethane\/methanol\/isopropanol\/water (9:6:1:4, <jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) system and RP\u2010HPLC. The structures of the isolates were determined utilising ESI\u2010Q\u2010TOF\u2010MS, one\u2010dimensional and two\u2010dimensional NMR and optical rotation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HPCCC on enriched saponin extract of <jats:styled-content style=\"fixed-case\"><jats:italic>C. japonica<\/jats:italic><\/jats:styled-content> yielded four saponin fractions in the order of the number of sugars attached to the triterpene aglycone, and preparative RP\u2010HPLC on each saponin fraction led to the isolation of nine novel saponins, namely camoreoside A\u2013I, along with six known ones.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study indicates that combination of HPLC\u2010ESI\u2010Q\u2010TOF\u2010MS analysis and HPCCC coupled with RP\u2010HPLC are excellent tools for discovering saponins from natural sources.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2808","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T01:26:36Z","timestamp":1543281996000},"page":"226-236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Separation of nine novel triterpene saponins from <scp><i>Camellia japonica<\/i><\/scp> seeds using high\u2010performance countercurrent chromatography and reversed\u2010phase high\u2010performance liquid chromatography"],"prefix":"10.1002","volume":"30","author":[{"given":"Taewoong","family":"Rho","sequence":"first","affiliation":[{"name":"College of Pharmacy and Integrated Research Institute of Pharmaceutical Science The Catholic University of Korea  Bucheon Gyeonggi Province Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo\u2010Jung","family":"Choi","sequence":"additional","affiliation":[{"name":"College of Pharmacy and 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