{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T02:35:54Z","timestamp":1771209354404,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T00:00:00Z","timestamp":1553644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005407","name":"Food and Health Bureau","doi-asserted-by":"publisher","award":["HMRF Project No. 11121841"],"award-info":[{"award-number":["HMRF Project No. 11121841"]}],"id":[{"id":"10.13039\/501100005407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The Chinese medicine formulation, tumour\u2010shrinking decoction (TSD, FM1523), which consists of 15 natural medicines, is used for uterine fibroids (UFs) therapy and possesses excellent clinical therapeutic effect.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To develop a sensitive and validated analytical method for the simultaneous quantification of four crucial bioactive compounds including isorhamnetin\u20103\u2010\n                      <jats:italic>O<\/jats:italic>\n                      \u2010neohesperidoside, curcumin, peimine and tetrahydropalmatine in the principal formulation of this decoction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>An ultra\u2010performance liquid chromatography coupled tandem mass spectrometry (UPLC\u2010MS\/MS) with an electrospray ionisation (ESI) source in multiple reaction monitoring (MRM) mode was conducted to investigate these bioactive compounds in the TSD. The chromatographic separation was performed on a C18 column when the flow rate was adjusted at 0.2\u00a0mL\/min with gradient elution of acetonitrile\u2013water with 0.1% formic acid. Accelerated solvent extraction (ASE) method with higher extraction efficiency was employed for TSD sample pre\u2010treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The linearity, limit of detection (LOD) and limit of quantification (LOQ) were determined for this analytical method. The mean recoveries of the compounds were determined between 100.23% and 104.02% with satisfactory relative standard deviation (RSD) in the ranges of 2.65% to 3.81%. The precision was evaluated by intra\u2010day and inter\u2010day tests, which revealed RSD within the ranges of 1.21% to 2.14% and 1.24% to 2.32%, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The bioactive compounds of TSD samples were successfully quantified via UPLC\u2010MS\/MS with MRM mode. This study could help to evaluate the pharmacokinetic study of TSD during clinical applications and present a facile strategy for quantifying bioactive compounds in traditional Chinese Medicine decoction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.2827","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T19:50:06Z","timestamp":1553716206000},"page":"447-455","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Application of UPLC\u2010MS\/MS to simultaneously detect four bioactive compounds in the tumour\u2010shrinking decoction (FM1523) for uterine fibroids treatment"],"prefix":"10.1002","volume":"30","author":[{"given":"Issac Chi\u2010Chung","family":"Cheng","sequence":"first","affiliation":[{"name":"School of Chinese Medicine, LKS Faculty of Medicine The University of Hong Kong  Pokfulam Hong 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