{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T10:42:41Z","timestamp":1776422561022,"version":"3.51.2"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T00:00:00Z","timestamp":1552262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673637"],"award-info":[{"award-number":["81673637"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Calcium channel blockers (CCBs) are currently the most commonly used drugs for the treatment of hypertension. Moutan Cortex (MC), a traditional Chinese herb, has been found to have an anti\u2010hypertensive effect. However, its potential mechanisms in the regulation of intracellular calcium concentration ([Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      ) remain poorly understood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The main objective of this work was to identify the potential calcium antagonists from MC and study their molecular mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Ultra\u2010high performance liquid chromatography\u2010quadrupole\u2010time\u2010of\u2010fight\u2010mass spectrometry (UHPLC\u2010QTOF\u2010MS) analysis combined with a dual\u2010luciferase reporter assay was utilised to systematically screen the calcium antagonistic active ingredients in the methanol extract of MC. Additionally, the molecular mechanism of these compounds was further studied using live\u2010cell imaging analysis with the calcium ion (Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ) probe dye fluo\u20104\/AM to monitor changes in [Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Three monoterpenoids (paeoniflorin, benzoylpaeoniflorin and mudanpioside C), one phenolic acid (paeonol) and one gallotannin (1,2,3,4,6\u2010\n                      <jats:italic>O<\/jats:italic>\n                      \u2010pentagalloylglucose) were screened out as potential calcium antagonists in MC. Among them, the calcium antagonistic activity of benzoylpaeoniflorin, mudanpioside C and 1,2,3,4,6\u2010\n                      <jats:italic>O<\/jats:italic>\n                      \u2010pentagalloylglucose is first reported. Additionally, paeoniflorin, benzoylpaeoniflorin, mudanpioside C and paeonol can effectively block voltage\u2010operated Ca\n                      <jats:sup>2+<\/jats:sup>\n                      channels (VOCCs) to exert calcium antagonism, while 1,2,3,4,6\u2010\n                      <jats:italic>O<\/jats:italic>\n                      \u2010pentagalloylglucose plays a role in blocking inositol 1,4,5\u2010trisphosphate receptors (IP3Rs).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This work indicated that the anti\u2010hypertensive efficacy of MC acted through multiple components selectively antagonising multiple cell signalling pathways to regulate [Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      . Furthermore, they could be considered as a reference standard for controlling the quality of Chinese medicinal materials.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.2828","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T02:08:30Z","timestamp":1552356510000},"page":"456-463","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Searching for calcium antagonists for hypertension disease therapy from Moutan Cortex, using bioactivity integrated UHPLC\u2010QTOF\u2010MS"],"prefix":"10.1002","volume":"30","author":[{"given":"Yujie","family":"Lu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research Nankai University  Tianjin P. R. 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