{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T23:15:26Z","timestamp":1778541326666,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T00:00:00Z","timestamp":1564531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2017YFD0201402"],"award-info":[{"award-number":["2017YFD0201402"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81173487"],"award-info":[{"award-number":["81173487"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81373904"],"award-info":[{"award-number":["81373904"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673535"],"award-info":[{"award-number":["81673535"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2020,3]]},"abstract":"Abstract<\/jats:title>\n \n Introduction<\/jats:title>\n \n Dioscorea<\/jats:italic>\n species, which contain abundant steroidal saponins, have been used as folk medicines or raw materials to synthesise steroid drugs.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n To establish a rapid chemotaxonomic method that will comprehensively resolve confusions about genetic relationships of genus\n Dioscorea<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n A comprehensive strategy using ultra\u2010high\u2010performance liquid chromatography coupled with quadrupole time\u2010of\u2010flight tandem mass spectrometry (UHPLC\u2010QTOF\u2010MS\/MS) was firstly proposed to evaluate the chemotaxonomy of 12 species (27 taxa) from China by hierarchical cluster analysis (HCA) based on the variations of the identified metabolites.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Twenty\u2010eight secondary metabolites (mainly steroidal saponins) were identified. The MS\n \n n<\/jats:italic>\n <\/jats:sup>\n fragmentation patterns of DA (a new acetylated steroidal saponin at C\u20107 position) were firstly reported. Moreover, eight major steroidal saponins were further quantified simultaneously by UPLC\u2010QTOF\u2010MS method. According to HCA results,\n \n D. bulbifera<\/jats:italic>\n <\/jats:styled-content>\n L. was distinguished with species of sect.\n Stenophora<\/jats:italic>\n Uline for pennogenin\u2010type steroidal saponins.\n Dioscorea zingiberensis<\/jats:italic>\n exhibited far distance from other members of sect.\n Stenophora<\/jats:italic>\n Uline for two unique saponins.\n Dioscorea banzhuana<\/jats:italic>\n may be reclassified into sect.\n Stenophora<\/jats:italic>\n .\n Dioscorea<\/jats:italic>\n \n nipponica<\/jats:italic>\n <\/jats:styled-content>\n subsp.\n rosthornii<\/jats:italic>\n and\n D. collettii<\/jats:italic>\n var.\n hypoglauca<\/jats:italic>\n might be separated from their original subspecies\/varieties as new species, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The chemotaxonomic method was successfully applied in the study of genetic relationships of\n Dioscorea<\/jats:italic>\n species. This study not only enhanced the understanding of chemical constituents, but also laid basic theoretical foundations for the rational utilisation and chemotaxonomy of genus\n Dioscorea<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/pca.2876","type":"journal-article","created":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T04:19:56Z","timestamp":1564546796000},"page":"164-182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Chemotaxonomic studies of 12\n Dioscorea<\/i>\n species from China by UHPLC\u2010QTOF\u2010MS\/MS analysis"],"prefix":"10.1002","volume":"31","author":[{"given":"Xuejiao","family":"Li","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory for Modern Drug Delivery & High\u2010Efficiency, School of Pharmaceutical Science and Technology Tianjin University Tianjin 300072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine Tianjin 300193 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8801-5562","authenticated-orcid":false,"given":"Jiachen","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Food Science Tianjin University of Commerce Tianjin 300134 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory for Modern Drug Delivery & High\u2010Efficiency, School of Pharmaceutical Science and Technology Tianjin University Tianjin 300072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengcheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory for Modern Drug Delivery & High\u2010Efficiency, School of Pharmaceutical Science and Technology Tianjin University Tianjin 300072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory for Modern Drug Delivery & High\u2010Efficiency, School of Pharmaceutical Science and Technology Tianjin University Tianjin 300072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuli","family":"Man","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology Tianjin University of Science & Technology Tianjin 300457 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0050-501X","authenticated-orcid":false,"given":"Wenyuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory for Modern Drug Delivery & High\u2010Efficiency, School of Pharmaceutical Science and Technology Tianjin University Tianjin 300072 China"},{"name":"Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine Tianjin 300193 China"},{"name":"Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology Tianjin University of Science & Technology Tianjin 300457 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,31]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1360330"},{"issue":"3","key":"e_1_2_5_3_1","first-page":"315","article-title":"Phylogeny of Dioscorea sect. 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