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The hexane extracts were analyzed by GC\u2010MS. Carbon\u201013 (<jats:sup>13<\/jats:sup>C)\u2010NMR analyzes of all extracts were performed. In both analyses, a biomarker was obtained.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The hexane extracts were determined as palmitic acid, arachidic acid, behenic acid, and linolenic acid as the main components. As a result of <jats:sup>13<\/jats:sup>C\u2010NMR analyzes, in hexane, chloroform, and ethylacetate the extracts detected were palmitic acid, arachidic acid, behenic acid, and linolenic acid. <jats:sc>d<\/jats:sc>\u2010Pinitol was obtained using <jats:sup>13<\/jats:sup>C\u2010NMR analyzes with <jats:italic>n<\/jats:italic>\u2010butanol and methanol extracts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrated that <jats:sc>d<\/jats:sc>\u2010pinitol is a biomarker for the endemic six <jats:italic>Astragalus<\/jats:italic> subsp.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2896","type":"journal-article","created":{"date-parts":[[2020,1,15]],"date-time":"2020-01-15T02:15:15Z","timestamp":1579054515000},"page":"306-313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Metabolomics of endemic six <i>Astragalus<\/i> species by combined NMR and GC\u2010MS analysis"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7668-6286","authenticated-orcid":false,"given":"Ayse","family":"Sahin Yaglioglu","sequence":"first","affiliation":[{"name":"Department of Chemistry Cankiri Karatekin Universitesi  Cankiri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat","family":"Temirturk","sequence":"additional","affiliation":[{"name":"Department of Chemistry Cankiri Karatekin Universitesi  Cankiri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emic","family":"Ugur","sequence":"additional","affiliation":[{"name":"Department of Chemistry Cankiri Karatekin Universitesi  Cankiri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melda","family":"Dolarslan","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Biomolecular Sciences Liverpool John Moores University  Liverpool UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibrahim","family":"Demirtas","sequence":"additional","affiliation":[{"name":"Department of Chemistry Cankiri Karatekin Universitesi  Cankiri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0486141"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1013713905833"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2004.06.015"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.53.105"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2007.12.001"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.03.124"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-006-9031-3"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-008-9091-7"},{"key":"e_1_2_6_10_1","unstructured":"DavisPH.Flora of Turkey and the East Aegean Islands. 7. 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