{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:34:50Z","timestamp":1761093290992,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,12,21]],"date-time":"2019-12-21T00:00:00Z","timestamp":1576886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"PRODEP","award":["2019"],"award-info":[{"award-number":["2019"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Annona purpurea<\/jats:italic><\/jats:styled-content> is a species known in Mexico as \u201ccabeza de negro\u201d. In folk medicine <jats:styled-content style=\"fixed-case\"><jats:italic>A. purpurea<\/jats:italic><\/jats:styled-content> root is used to treat patients with kidney diseases and cancer. Our recent studies demonstrated that this species contains five acetogenins named annopurpuricins A\u2013E, which are active against tumoural cell lines in a subnanomolar range.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop an analytical method using a high\u2010performance liquid chromatography diode array detector (HPLC\u2010DAD) to quantify annopurpuricins A\u2013E in different <jats:styled-content style=\"fixed-case\"><jats:italic>A. purpurea<\/jats:italic><\/jats:styled-content> root samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>To quantify the five annopurpuricins A\u2013E a sample treatment was carried out, which consisted of fractionation by means of cold and hot maceration; using solvents of ascending polarity: hexane, dichloromethane, methanol and water. The resulting extracts were subject to HPLC\u2010DAD analysis. The optimised chromatographic separation on a XBRIDGE C18 column achieved separation of all compounds in around 30 min.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The developed method was validated according to ICH (International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use) validation guide. The developed analytical method was found fast, economic, robust, sensitive, linear and precise. The dichloromethane extract of <jats:styled-content style=\"fixed-case\"><jats:italic>A. purpurea<\/jats:italic><\/jats:styled-content> contains annopurpuricin A in quantities 2\u2010 to 25\u2010fold higher than annopurpuricins B\u2013E. This optimised method identified and quantified five annopurpuricins, highly bioactive molecules, in <jats:styled-content style=\"fixed-case\"><jats:italic>A. purpurea<\/jats:italic><\/jats:styled-content> root.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The fingerprint of the dichloromethane extracts of <jats:styled-content style=\"fixed-case\"><jats:italic>A. purpurea<\/jats:italic><\/jats:styled-content> was obtained at 210 nm. The results analysis allowed to quantify annopurpuricins A\u2013E that are present in different collection batches of medium polarity extracts. After data analysis, annopurpuricin A could be establish as the metabolite marker of the root of the species.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2910","type":"journal-article","created":{"date-parts":[[2019,12,21]],"date-time":"2019-12-21T04:25:38Z","timestamp":1576902338000},"page":"472-479","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["HPLC\u2010DAD method for the detection of five annopurpuricins in root samples of <scp><i>Annona purpurea<\/i><\/scp>"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4685-3095","authenticated-orcid":false,"given":"Gustavo A.","family":"Hern\u00e1ndez\u2010Fuentes","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas Universidad de Colima  Colima Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2252-5890","authenticated-orcid":false,"given":"Ana 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