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No international certified standard method is available for kinsenoside analysis as well as extraction and preservation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To develop a more accurate analytical method of kinsenoside. The effects of extraction and drying methods of <jats:styled-content style=\"fixed-case\"><jats:italic>A. roxburghii<\/jats:italic><\/jats:styled-content> on kinsenoside efficiency were investigated for the first time, as well as to examine the kinsenoside stability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>The amino (NH<jats:sub>2<\/jats:sub>) and AQ\u2010C<jats:sub>18<\/jats:sub> columns for detecting kinsenoside extract was systematically compared by high\u2010performance liquid chromatography evaporative light\u2010scattering detector (HPLC\u2010ELSD) and HPLC\u2010diode\u2010array detector (DAD), respectively. Kinsenoside, its epimer goodyeroside A and the degradation product during preservation were identified through HPLC\u2010electrospray ionization mass spectrometry (ESI\u2010MS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An accurate method of kinsenoside detection by HPLC\u2010ELSD with dual columns of NH<jats:sub>2<\/jats:sub> and AQ\u2010C<jats:sub>18<\/jats:sub> was established. The ratio of <jats:italic>C<\/jats:italic><jats:sub>goodyeroside A<\/jats:sub> to <jats:italic>C<\/jats:italic><jats:sub>kinsenoside<\/jats:sub> (<jats:italic>Y<\/jats:italic>) was determined using the AQ\u2010C<jats:sub>18<\/jats:sub> column method. The concentration detected by the NH<jats:sub>2<\/jats:sub> column was multiplied by 1\/(1 + <jats:italic>Y<\/jats:italic>) as the corrected result. Using this novel method, the average deviations were reduced by 7.64%. Moreover, the efficiency of kinsenoside extraction with water was almost twice that of extraction with ethanol. Freeze drying also led to a higher extraction efficiency (38.47% increase) than hot\u2010air drying did. Furthermore, the degradation of kinsenoside extract exceeded 70% when stored at 37\u2009\u00b0C for 3 months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides a reliable experimental method and theoretical basis for the quality control of kinsenoside from <jats:styled-content style=\"fixed-case\"><jats:italic>A. roxburghii<\/jats:italic><\/jats:styled-content>, as well as other glycosides.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2933","type":"journal-article","created":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T09:39:24Z","timestamp":1585215564000},"page":"700-710","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Detecting kinsenoside from <i>Anoectochilus roxburghii<\/i> by HPLC\u2010ELSD with dual columns of NH<sub>2<\/sub> and AQ\u2010C<sub>18<\/sub>"],"prefix":"10.1002","volume":"31","author":[{"given":"Mi","family":"Wei","sequence":"first","affiliation":[{"name":"Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology Huazhong University of Science and Technology  Wuhan 430074 China"},{"name":"Key Laboratory for Quality Control of Characteristic Fruits and Vegetables of Hubei Province, College of Life Science and Technology Hubei Engineering University  Xiaogan 432000 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemin","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology Huazhong University of Science and Technology  Wuhan 430074 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwen","family":"Yi","sequence":"additional","affiliation":[{"name":"Institute of Resource 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