{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:07:25Z","timestamp":1790795245895,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T00:00:00Z","timestamp":1594166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 81603336"],"award-info":[{"award-number":["No. 81603336"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The diversity and complexity of components are important reasons for the unstable efficacy and safety of Chinese materia medica (CMM) in quality control. The good and stable quality control may be related to the quality markers with structural composition of multi\u2010components.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>In the present study, we take <jats:styled-content style=\"fixed-case\"><jats:italic>Eclipta prostrata<\/jats:italic><\/jats:styled-content> L. as a representative example. The 11 samples were collected from the different areas in China, and the discrepancy in bioactivity and chemical composition of these samples were compared. DOX (doxorubicin hydrochloride)\u2010induced ICR mice were established for <jats:italic>in vivo<\/jats:italic> nephrotic syndrome experiments. The biochemical indicators including 24\u2010h urine protein, triglyceride (TG), etc. were measured and the pathological change of kidney tissue was observed. MPC\u20105 cells damage model was induced to compare the difference of these samples in bio\u2010activity. High\u2010performance liquid chromatography (HPLC) method for 11 EEP (extract of <jats:styled-content style=\"fixed-case\"><jats:italic>Eclipta prostrata<\/jats:italic><\/jats:styled-content> L.) samples were performed to analyse the content of the quality markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>In vivo<\/jats:italic> experiments, EEP could mitigate the content or activity of urine protein, TG, etc. compared with the positive group (TG content was 2.53 \u00b1 0.39 mmol\/L, urinary protein quantification on 35th day was 16.79 \u00b1 2.32 mg). <jats:italic>In vitro<\/jats:italic> experiments, CCE (coumestans component of Ecliptae) and EEP had equivalent effects on biochemical indicators such as cell viability, etc. According to the HPLC analysis, the content of wedelolactone was 45.88% and demethylwedelolactone was 23.74% in CCE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The CCE with a ratio of 2:1 can be considered as a quality marker of <jats:styled-content style=\"fixed-case\"><jats:italic>Eclipta prostrata<\/jats:italic><\/jats:styled-content> L.. This research may provide a perspective for quality control of CMM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.2969","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T19:46:11Z","timestamp":1594237571000},"page":"968-981","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The combination of HPLC and biological analysis to determine the quality markers and its structural composition of <scp><i>Eclipta prostrata<\/i><\/scp> L."],"prefix":"10.1002","volume":"31","author":[{"given":"Long","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenzi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing P. 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