{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T09:21:15Z","timestamp":1780564875409,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T00:00:00Z","timestamp":1607644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Medicinal plants have been used in healthcare since time immemorial, as have their therapeutic activities and the production of plant\u2010based medicines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study aims to use gene\u2010targeted molecular markers for genetic diversity analysis of 16 medicinal plants. Besides, phytochemical analysis antibacterial and antifungal activities of some medicinal plant extracts commonly used in Egypt are compared to major compounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>DNA\u2010based classification of 16 medicinal species using Conserved DNA\u2010Derived Polymorphism (CDDP) and Start Codon Targeted (SCoT) primers. Three species representing three orders (<jats:styled-content style=\"fixed-case\"><jats:italic>Pelargonium graveolens<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>Matricaria chamomilla<\/jats:italic><\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\"><jats:italic>Hyoscyamus muticus<\/jats:italic><\/jats:styled-content> were analysed [high\u2010performance liquid chromatography (HPLC), gas chromatography\u2010mass spectrometry (GC\u2010MS)] and evaluated for their antibacterial and antifungal activities against (<jats:styled-content style=\"fixed-case\"><jats:italic>Escherichia coli<\/jats:italic><\/jats:styled-content> O157: H7 ATCC 93111, <jats:italic>Salmonella typhimurium<\/jats:italic> ATCC 14028, Methicillin\u2010resistant <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content> (MRSA) ATCC 43300, <jats:italic>Bacillus ceruse<\/jats:italic> ATCC 33018, and <jats:italic>Sclerotinia sclerotiorum<\/jats:italic> in comparison with some of their antimicrobial components.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results revealed 309 and 349 polymorphic bands with 100% polymorphism. Among them, 51 and 57 were unique loci for CDDP and SCoT, respectively. The 16 species were categorised into three groups depending on the similarity matrix. The results of antibacterial and antifungal activities revealed that <jats:italic>Pelargonium<\/jats:italic> oil showed significant antifungal and antibacterial activities against the tested pathogens. Gallic acid severely reduced all tested bacteria's growth, but atropine severely reduced the growth of the <jats:italic>B. ceruse<\/jats:italic> only. Molecular modelling revealed their activity against sclerotium development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The gene\u2010targeted marker techniques were highly useful tools for the classification of the 16 medicinal plant species, despite displaying high similarities at morphological and phytochemical analyses but, have antifungal and antibacterial activities.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3018","type":"journal-article","created":{"date-parts":[[2020,12,12]],"date-time":"2020-12-12T01:45:05Z","timestamp":1607737505000},"page":"724-739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Gene\u2010targeted molecular phylogeny, phytochemical analysis, antibacterial and antifungal activities of some medicinal plant species cultivated in Egypt"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4129-4442","authenticated-orcid":false,"given":"Hanaa S.","family":"Omar","sequence":"first","affiliation":[{"name":"Genetics Department, Faculty of Agriculture Cairo University  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1272-6453","authenticated-orcid":false,"given":"Tarek R.","family":"Elsayed","sequence":"additional","affiliation":[{"name":"Microbiology Department, Faculty of Agriculture Cairo University  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nour El\u2010Houda A.","family":"Reyad","sequence":"additional","affiliation":[{"name":"Plant Pathology Department, Faculty of Agriculture Cairo University  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Israa. M.","family":"Shamkh","sequence":"additional","affiliation":[{"name":"Chemo Informatics Lab, Faculty of Agriculture Cairo University, Research Park, CURP  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5612-8546","authenticated-orcid":false,"given":"Mohamed S.","family":"Sedeek","sequence":"additional","affiliation":[{"name":"Pharmacognosy Department, Faculty of Pharmacy Cairo University  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nep067"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/283484"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-38334-4"},{"issue":"2","key":"e_1_2_5_5_1","first-page":"200","article-title":"Molecular markers associated with genetic diversity of some medicinal plants in Sinai","volume":"5","author":"Domyati FM","year":"2011","journal-title":"J Med Plant Res"},{"issue":"5","key":"e_1_2_5_6_1","first-page":"689","article-title":"Traditional medicinal plants research in Egypt: Studies of antioxidant and anticancer activities","volume":"6","author":"Aboul\u2010Enein AM","year":"2012","journal-title":"J Med Plant Res"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-017-9739-1"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2007.04.008"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2010.519390"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejsobi.2013.10.008"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10681-010-0286-9"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.3844\/ajassp.2012.752.758"},{"key":"e_1_2_5_13_1","unstructured":"FAO.Measurement of domestic animal diversity. Food and Agriculture Organization of the United Nations (FAO): Rome 2004."},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/16878507.2019.1697079"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11105-008-0060-5"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2016.12.028"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1556\/CRC.2014.0010"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2013.08.027"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.7550\/rmb.39066"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky998"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.27.863"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221-1691(12)60465-1"},{"key":"e_1_2_5_23_1","first-page":"1","article-title":"Chemical composition and potentiation of insecticidal and fungicidal activities of Citrus trifoliata L. fruits essential oil against Spodoptera littoralis, Fusarium oxysporum and Fusarium solani via nano\u2010cubosomes","author":"Abdel\u2010Kawy MA","year":"2019","journal-title":"Nat Prod Res"},{"issue":"374","key":"e_1_2_5_24_1","first-page":"43","article-title":"Comparative antibacterial and antifungal effects of some phenolic compounds","volume":"93","author":"Aziz NH","year":"1998","journal-title":"Microbios"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2014.05.021"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11105-009-0118-z"},{"key":"e_1_2_5_27_1","first-page":"223","article-title":"Nouvelle recherches Sur la distribution florale","volume":"44","year":"1908","journal-title":"Bull Soc Vaud Sci Nat"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/45.4_ts.493"},{"key":"e_1_2_5_29_1","first-page":"413","article-title":"Molecular characterization of Giloe (Tinospora cordifolia Willd. Miers ex hook. F. and Thoms.) accessions using start codon targeted (SCoT) markers","volume":"3","author":"Paliwal R","year":"2013","journal-title":"Int J Med Aromat Plan Theory"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2020.03.007"},{"issue":"2","key":"e_1_2_5_31_1","first-page":"511","article-title":"Antimicrobial and antifungal activity of Pelargonium roseum essential oils","volume":"4","author":"Carmen G","year":"2014","journal-title":"Adv Pharm Bull"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.13053"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2012.0244"},{"key":"e_1_2_5_34_1","first-page":"268","article-title":"Lipids and other natural products","author":"Salisbury FB","year":"1986","journal-title":"Plant Physiol"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24183250"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-19-0682"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2016-0091"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04390.x"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.py.23.090185.000525"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/13102818.2017.1407255"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.3018","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pca.3018","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.3018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T09:42:14Z","timestamp":1761039734000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.3018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,11]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1002\/pca.3018"],"URL":"https:\/\/doi.org\/10.1002\/pca.3018","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"value":"0958-0344","type":"print"},{"value":"1099-1565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,11]]},"assertion":[{"value":"2020-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}