{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:34:08Z","timestamp":1789166048315,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Dried extracts of <jats:styled-content style=\"fixed-case\"><jats:italic>Piper methysticum<\/jats:italic><\/jats:styled-content> G. Forst, also known as kava, has been widely used due to its anxiolytic and sedative properties. In order to assure the quality of these extracts, it is essential to accurately quantify kavalactones, known as the active principle.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To develop and validate an analytical method for the simultaneous quantification of six major kavalactones (kavain, dihydrokavain, methysticin, dihydromethysticin, yangonin and demethoxyyangonin) in kava extracts, comparing multi\u2010standards and single standard validation approaches.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Separation was performed using a C18 column, water\/methanol\/acetonitrile\/2\u2010propanol (66:07:09:18\u00a0<jats:italic>v\/v\/v\/v<\/jats:italic>) and detection at 245 and 350\u00a0nm. A full method validation was performed, employing analytical standards for each compound. Commercial kava dried extracts were assayed and the results obtained using the method validated for six kavalactone standards were compared with those obtained when only kavain was used as standard.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Baseline resolution for all kavalactones was obtained in short run time (15\u00a0min). Although the total kavalactone content varied between samples, a similar distribution profile was observed. When the method validated with all six analytical standards was compared to the calibration using only kavain standard, kavalactone contents were considerably different (from 7.57 to 36.53%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The obtained results demonstrate the importance of a validated method using individual kavalactone standards for the effective quality control of kava extracts. In a next step, the method needs to be adapted to also include flavokavin B (FKB), as an important authentication marker to distinguish between the accepted variety \u201cnoble Kava\u201d and the toxic \u201ctwo\u2010day Kava\u201d.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3019","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T23:45:49Z","timestamp":1607384749000},"page":"740-748","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Simultaneous quantitation of kavalactones in kava dry extracts: comparison of multi\u2010standards and single standard validation approaches"],"prefix":"10.1002","volume":"32","author":[{"given":"Juliana Veloso","family":"Ferreira","sequence":"first","affiliation":[{"name":"Departamento de Produtos Farmac\u00eauticos, Faculdade de Farm\u00e1cia Universidade Federal de Minas Gerais  Belo Horizonte MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella Campolina","family":"Pierotte","sequence":"additional","affiliation":[{"name":"Departamento de Produtos Farmac\u00eauticos, Faculdade de Farm\u00e1cia Universidade Federal de Minas Gerais  Belo Horizonte MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerson Ant\u00f4nio","family":"Pianetti","sequence":"additional","affiliation":[{"name":"Departamento de Produtos Farmac\u00eauticos, Faculdade de Farm\u00e1cia Universidade Federal de Minas Gerais  Belo Horizonte MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1349-9413","authenticated-orcid":false,"given":"Isabela Costa","family":"C\u00e9sar","sequence":"additional","affiliation":[{"name":"Departamento de Produtos Farmac\u00eauticos, Faculdade de Farm\u00e1cia Universidade Federal de Minas Gerais  Belo Horizonte MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,7]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Kava \u2013 from ethnology to pharmacology","author":"Singh YN","year":"2004"},{"key":"e_1_2_6_3_1","volume-title":"Buveurs de Kava","author":"Sim\u00e9oni P","year":"2014"},{"key":"e_1_2_6_4_1","unstructured":"European Medicines Agency.Assessment report on Piper methysticum G. 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