{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:50:50Z","timestamp":1791003050707,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T00:00:00Z","timestamp":1635984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Hypericum perforatum<\/jats:italic><\/jats:styled-content> L. (St. John's wort) is a medicinally important member of Hypericaceae. Many pharmacological activities have been mostly attributed to its hyperforin, hypericin and\/or hyperoside contents. Therefore, qualitative and quantitative determinations of these ingredients are essential to justify the beneficial effects of St. John's wort on health. In the European Pharmacopoeia, the TLC and HPLC methods were given for this purpose. High performance thin layer chromatography (HPTLC) has recently become increasingly used as a suitable technique for analysing herbal drugs. This study aims to develop new and validated HPTLC methods to analyse these active components in different <jats:italic>Hypericum<\/jats:italic> spp. to find other suitable species to replace the official plant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three different mobile phases were developed: <jats:italic>n<\/jats:italic>\u2010hexane\u2010ethyl acetate (8:2) for hyperforin analysis, toluene\u2010chloroform\u2010ethyl acetate\u2010formic acid (8:5:3.5:0.6) for hypericin analysis and ethyl acetate\u2010formic acid\u2010acetic acid\u2010water (15:2:2:1) for hyperoside analysis. These newly developed and validated HPTLC systems were further applied to determine their concentrations in different <jats:italic>Hypericum<\/jats:italic> species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hyperforin concentration was found between 6.40 to 26.40\u2009mg\/g only in <jats:italic>H. triquetrifolium<\/jats:italic>, <jats:styled-content style=\"fixed-case\"><jats:italic>H. scabrum<\/jats:italic><\/jats:styled-content> and two <jats:styled-content style=\"fixed-case\"><jats:italic>H. perforatum<\/jats:italic><\/jats:styled-content> samples; hypericin was detected between 0.81 and 1.41\u2009mg\/g only in <jats:italic>H. bithynicum<\/jats:italic>, <jats:italic>H. perfoliatum<\/jats:italic>, <jats:italic>H. triquetrifolium<\/jats:italic> and two <jats:styled-content style=\"fixed-case\"><jats:italic>H. perforatum<\/jats:italic><\/jats:styled-content> samples; and hyperoside was identified in all tested specimens ranging from 1.01 to 9.73\u2009mg\/g. The new HPTLC methods developed and validated in the present study may ensure reliable results for the qualification and quantification of hyperforin, hypericin and hyperoside contents in <jats:italic>Hypericum<\/jats:italic> species.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3093","type":"journal-article","created":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T08:00:23Z","timestamp":1636012823000},"page":"355-364","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of new and validated HPTLC methods for the qualitative and quantitative analysis of hyperforin, hypericin and hyperoside contents in <i>Hypericum<\/i> species"],"prefix":"10.1002","volume":"33","author":[{"given":"Esra","family":"Sa\u00e7\u0131c\u0131","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Yeditepe University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1348-6033","authenticated-orcid":false,"given":"Erdem","family":"Yesilada","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Yeditepe University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2005.07.008"},{"key":"e_1_2_8_3_1","volume-title":"EdQm","author":"European Pharmacopoeia","year":"2019"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1211\/0022357011775910"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200645050-00002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2006.08.017"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2005.12.027"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404301"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.12.030"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms11020562"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.10.011"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2005.06.009"},{"key":"e_1_2_8_13_1","first-page":"1","article-title":"Advanced research on the antidepressant effect of flavonoids","author":"Gong J","year":"2014","journal-title":"Curr Opin Complement Alternat Med"},{"key":"e_1_2_8_14_1","volume-title":"High\u2010Performance Thin\u2010Layer Chromatography in Food Analysis","author":"Jaiswal PK","year":"2010"},{"key":"e_1_2_8_15_1","volume-title":"High\u2010Performance Thin Layer Chromatography for the Analysis of Medicinal Plants","author":"Reich E","year":"2006"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2013.852546"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2013.872675"},{"key":"e_1_2_8_18_1","first-page":"303","article-title":"Developing a new high performance thin layer chromatography method for quantitative estimation of hyperforin in Hypericum perforatum","volume":"4","author":"Tewari N","year":"2008","journal-title":"Phcog Mag"},{"key":"e_1_2_8_19_1","first-page":"30","article-title":"Fingerprint HPTLC analysis of marketed botanicals","volume":"3","author":"Nicoletti M","year":"2012","journal-title":"Pharmacol Online"},{"key":"e_1_2_8_20_1","first-page":"70","volume-title":"Plant Drug Analysis \u2010 A Thin Layer Chromatography Atlas","author":"Wagner H","year":"2001"},{"key":"e_1_2_8_21_1","first-page":"217","volume-title":"Studies in Natural Product Chemistry","author":"Nicoletti M","year":"2012"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-695X2011005000131"},{"key":"e_1_2_8_23_1","first-page":"41","article-title":"HPTLC chromatographic polyphenolic fingerprints of plant species from Eastern Europe","volume":"3","author":"Bubueanu C","year":"2016","journal-title":"Malays J Med Biol Res"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2015.1028295"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/385473"},{"key":"e_1_2_8_26_1","volume-title":"ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2 (R1)","author":"Quality Guidelines"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.01.059"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12711"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.03.048"},{"key":"e_1_2_8_30_1","first-page":"549","volume-title":"Herbal Medicines","author":"Barnes J","year":"2007"},{"key":"e_1_2_8_31_1","volume-title":"Monographs on Selected Medicinal Plants","author":"WHO","year":"2002"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2008.01.011"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112202"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules14020682"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2017.1298030"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.16-0170"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2017.12.012"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.3093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pca.3093","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.3093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T13:56:43Z","timestamp":1761055003000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.3093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,4]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1002\/pca.3093"],"URL":"https:\/\/doi.org\/10.1002\/pca.3093","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"value":"0958-0344","type":"print"},{"value":"1099-1565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,4]]},"assertion":[{"value":"2021-07-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}