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Methods of obtaining pure tomentosin are not sufficient for anticancer drug research.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study aims to develop a specific method to isolate tomentosin from <jats:styled-content style=\"fixed-case\"><jats:italic>I. viscosa<\/jats:italic><\/jats:styled-content> with high yield. It also aims to investigate the inhibitory effects of tomentosin on human carbonic anhydrase I (hCAI), human carbonic anhydrase II (hCAII), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), <jats:italic>\u03b1<\/jats:italic>\u2010glucosidase, and <jats:italic>\u03b1<\/jats:italic>\u2010amylase enzymes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Tomentosin was purified by a specific column chromatography method. The content of tomentosin in dichloromethane, dichloromethane by Soxhlet method, ethanol and ethanol by Soxhlet method extracts of <jats:styled-content style=\"fixed-case\"><jats:italic>I. viscosa<\/jats:italic><\/jats:styled-content> was determined by liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS). Half maximal inhibitory concentration (IC<jats:sub>50<\/jats:sub>) and inhibition constant (K<jats:sub>i<\/jats:sub>) values were calculated to determine <jats:italic>in vitro<\/jats:italic> enzyme inhibition effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Tomentosin was isolated in high yield (0.64%). The IC<jats:sub>50<\/jats:sub> and K<jats:sub>i<\/jats:sub> values for tomentosin were calculated as 5.00\u2009\u00b1\u20090.19 (<jats:italic>r<\/jats:italic> = 0.9688) and 4.62\u2009\u00b1\u20090.10\u00a0\u03bcM for hCAI, 5.40\u2009\u00b1\u20090.26 (<jats:italic>r<\/jats:italic> = 0.9677) and 5.22\u2009\u00b1\u20090.31\u2009\u03bcM for hCAII, 6.75\u2009\u00b1\u20090.208 (<jats:italic>r<\/jats:italic> = 0.9891) and 3.75\u2009\u00b1\u20090.27\u2009\u03bcM for AChE, 6.67\u2009\u00b1\u20090.307 (<jats:italic>r<\/jats:italic> = 0.9820) and 0.51\u2009\u00b1\u20090.11\u2009\u03bcM for BChE, 26.61\u2009\u00b1\u20090.236 (<jats:italic>r<\/jats:italic> = 0.9815) and 2.61\u2009\u00b1\u20090.71\u2009\u03bcM for <jats:italic>\u03b1<\/jats:italic>\u2010glucosidase and 26.89\u2009\u00b1\u20091.54 \u03bcM (<jats:italic>r<\/jats:italic> = 0.9670) for <jats:italic>\u03b1<\/jats:italic>\u2010amylase, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Tomentosin was isolated in high yield from the paste\u2010like extract of <jats:styled-content style=\"fixed-case\"><jats:italic>I. viscosa<\/jats:italic><\/jats:styled-content> compared to the positive controls, it was determined that tomentosin was weakly effective against hCAI, hCAII, AChE and BChE, but thoroughly effective against <jats:italic>\u03b1<\/jats:italic>\u2010glucosidase and <jats:italic>\u03b1<\/jats:italic>\u2010amylase. These results suggested that tomentosin has <jats:italic>\u03b1<\/jats:italic>\u2010glucosidase and <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibitor potential.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3114","type":"journal-article","created":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T03:53:47Z","timestamp":1646366027000},"page":"612-618","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["A new specific method for isolation of tomentosin with a high yield from <scp><i>Inula viscosa<\/i><\/scp> (L.) and determination of its bioactivities"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7653-6480","authenticated-orcid":false,"given":"Tuba","family":"Aydin","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy A\u011fr\u0131 \u0130brahim \u00c7e\u00e7en University  Agri 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