{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:31:53Z","timestamp":1790728313819,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T00:00:00Z","timestamp":1647993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073975"],"award-info":[{"award-number":["82073975"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Andrographolide active pharmaceutical ingredient (API) is a semidiurnal diterpene lactone with significant antipyretic, antiviral, anti\u2010inflammatory and anticancer activities. A large amount of andrographolide API could only be obtained by extraction from <jats:styled-content style=\"fixed-case\"><jats:italic>Andrographis paniculata<\/jats:italic><\/jats:styled-content>. Therefore, there may be related compounds, plant proteins and other impurities in andrographolide API.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>In order to improve the safety of andrographolide related preparations, it was necessary to clarify the impurities and improve the quality standard of andrographolide API.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The related compounds were identified by ultrahigh\u2010performance liquid chromatography with diode\u2010array detector quadrupole Orbitrap mass spectrometry (UHPLC\u2010DAD\u2010Q\/Orbitrap\u2010MS), and the residual proteins were determined by ultrafiltration combined with proteomics. The proteomics method included protein extraction, content determination, digestion, desalination and nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC\u2013MS\/MS) analysis. Then, MS results were compared with <jats:styled-content style=\"fixed-case\"><jats:italic>Andrographis paniculata<\/jats:italic><\/jats:styled-content> protein database by Peaks Studio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed that 32 related compounds were putatively identified, of which 30 impurities were identified for the first time. Seven residual proteins together with 11 highly suspected proteins were uniquely identified, including the T1UNN5_ANDPA protein with the highest intensity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study will provide useful information on the composition of andrographolide API, which is important for the quality control and clinical safety assurance of API and related prescriptions. Reasonable guidance will also be provided on the necessity of ultrafiltration in the production process of related injections.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3124","type":"journal-article","created":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T19:10:27Z","timestamp":1648062627000},"page":"735-745","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Proteomics and UHPLC\u2010DAD\u2010Q\/Orbitrap\u2010MS used to identify impurities in andrographolide"],"prefix":"10.1002","volume":"33","author":[{"given":"Shuangbing","family":"Deng","sequence":"first","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine, Jiangsu Collaborative Innovation Centre of Chinese Medicinal Resources Industrialisation, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae  Nanjing China"},{"name":"State Key Laboratory of Innovative Natural Medicine and TCM Injections Jiangxi Qingfeng Pharmaceutical Co., Ltd  Ganzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine, Jiangsu Collaborative Innovation Centre of Chinese Medicinal Resources Industrialisation, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Pharmaceutical University Guangdong Research Centre for Quality Engineering Technology of Traditional Chinese Medicine, Key Laboratory of Digitalised Quality Evaluation Technology of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaojiao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine, Jiangsu Collaborative Innovation Centre of Chinese Medicinal Resources Industrialisation, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Luo","sequence":"additional","affiliation":[{"name":"Guangdong Pharmaceutical University Guangdong Research Centre for Quality Engineering Technology of Traditional Chinese Medicine, Key Laboratory of Digitalised Quality Evaluation Technology of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Innovative Natural Medicine and TCM Injections Jiangxi Qingfeng Pharmaceutical Co., Ltd  Ganzhou 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0987-8317","authenticated-orcid":false,"given":"Hongyue","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine, Jiangsu Collaborative Innovation Centre of Chinese Medicinal Resources Industrialisation, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae  Nanjing 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