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High\u2010performance thin\u2010layer chromatography (HPTLC) coupled bioassay guided isolation of bioactive compounds has been improved within last few years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>A microchemical derivatised HPTLC\u2010coupled attenuated total reflectance\u2010Fourier\u2010transform infrared (ATR\u2010FTIR) and nuclear magnetic resonance (NMR) spectroscopy was employed for profiling <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibitor from the aerial part of <jats:italic>Asparagus racemosus<\/jats:italic> Willd.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Asparagus racemosus<\/jats:italic><\/jats:styled-content> Willd. aerial part extracted with different solvents (<jats:italic>n\u2010<\/jats:italic>hexane, chloroform, ethyl acetate, and methanol) and assayed to detect free radical scavengers and <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibitor by 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH) assay and starch\u2010iodine assay method, respectively. HPTLC\u2010coupled ATR\u2010FTIR and NMR spectroscopy was used to identify the <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibitor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Methanolic extract of <jats:styled-content style=\"fixed-case\"><jats:italic>A. racemosus<\/jats:italic><\/jats:styled-content> showed highest antioxidant activity (21.99\u2009\u03bcg GAE\/\u03bcL) where <jats:italic>n\u2010<\/jats:italic>hexane extract showed lowest antioxidant activity (5.87\u2009\u03bcg GAE\/\u03bcL). The <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibition was recorded as highest and lowest in ethyl acetate extract (13.13 AE\/\u03bcL) and <jats:italic>n\u2010<\/jats:italic>hexane extract (3.92 AE\/\u03bcL), respectively. The deep blue zone of <jats:italic>\u03b1<\/jats:italic>\u2010amylase sprayed TLC plate of extracts with <jats:italic>hR<\/jats:italic><jats:sub>F<\/jats:sub>\u2009=\u200972 analysed for ATR\u2010FTIR and NMR spectroscopy which revealed the presence of stigmasterol is responsible for <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present work establishes the <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibiting properties of <jats:styled-content style=\"fixed-case\"><jats:italic>A. racemosus<\/jats:italic><\/jats:styled-content> maintaining its use for the treatment of DM as a traditional medicine. Bioassay guided isolation through HPTLC\u2010coupled ATR\u2010FTIR and NMR spectroscopy offers an effective method for the exploration of bioactive compounds such as <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibitor from complex plant extracts.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3155","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T02:00:08Z","timestamp":1655863208000},"page":"1018-1027","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["High\u2010performance thin\u2010layer chromatography coupled attenuated total reflectance\u2010Fourier\u2010transform infrared and NMR spectroscopy\u2010based identification of <i>\u03b1<\/i>\u2010amylase inhibitor from the aerial part of <i>Asparagus racemosus<\/i> Willd"],"prefix":"10.1002","volume":"33","author":[{"given":"Priya","family":"Das","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gouhar Jahan","family":"Ashraf","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Baishya","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"},{"name":"Department of Tea Science University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarun Kumar","family":"Dua","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paramita","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gouranga","family":"Nandi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2229-1019","authenticated-orcid":false,"given":"Ranabir","family":"Sahu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,21]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Flora of the District Garhwal, North West Himalaya","author":"Gaur R","year":"1999"},{"key":"e_1_2_6_3_1","first-page":"2499","article-title":"Indian materia medica","volume":"3","author":"Kirtikar K","year":"1975","journal-title":"India"},{"issue":"9","key":"e_1_2_6_4_1","first-page":"408","article-title":"Asparagus racemosus\u2010an update","volume":"57","author":"Goyal R","year":"2003","journal-title":"Indian J Med Sci"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","unstructured":"AkbarS.Handbook of 200 medicinal plants: A comprehensive review of their traditional medical uses and scientific justifications.2020. doi:10.1007\/978\u20103\u2010030\u201016807\u20100","DOI":"10.1007\/978-3-030-16807-0"},{"issue":"3","key":"e_1_2_6_6_1","first-page":"742","article-title":"Shatavari (Asparagus racemosus Willd.): A review on its cultivation, morphology, phytochemistry and pharmacological importance","volume":"5","author":"Singla R","year":"2014","journal-title":"Int J Pharm Life Sci"},{"issue":"3","key":"e_1_2_6_7_1","first-page":"259","article-title":"Asparagus racemosus: for medicinal uses & pharmacological actions","volume":"4","author":"Hasan N","year":"2016","journal-title":"Int J Adv Res"},{"issue":"4","key":"e_1_2_6_8_1","first-page":"18","article-title":"Asparagus racemosus (Shatawari), phytoconstituents and medicinal importance, future source of economy by cultivation in Uttrakhand: A review","volume":"4","author":"Joshi RK","year":"2016","journal-title":"Inter J Herb Med"},{"key":"e_1_2_6_9_1","unstructured":"ShendeA.MEDICINAL PLANT ASPARAGUS RACEMOSUS: A BOON FOR WOMEN'S HEALTH. 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