{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T03:27:55Z","timestamp":1789183675374,"version":"build-2803163510"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T00:00:00Z","timestamp":1680566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008968","name":"National Research Council Sri Lanka","doi-asserted-by":"publisher","award":["NRC Grant 11\u2010107"],"award-info":[{"award-number":["NRC Grant 11\u2010107"]}],"id":[{"id":"10.13039\/100008968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2023,6]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>Nothapodytes nimmoniana<\/jats:italic> (Icacinaceae) is a rich source of Camptothecin (CPT), an anti\u2010cancer prodrug. Efficient extraction of CPT from various plant parts is crucial for better recovery of this pre\u2010drug.<\/jats:p><\/jats:sec>Objectives<\/jats:title>To investigate the distribution of CPT in plant parts and to compare the methods of extraction on CPT yield to evaluate how cellular localisation affects the efficiency of extraction methods.<\/jats:p><\/jats:sec>Methods<\/jats:title>Transverse sections of plant parts were observed under a ultraviolet (UV)\u2010fluorescence microscope for the fluorescence that the CPT molecule emits when exposed to UV radiation. Dried plant parts were extracted using 90% methanol with ultrasonic assistance, hot ethanol (61% ethanol at 60\u00b0C), and chloroform\u2013methanol (4:1, v\/v). The CPT in plant parts were detected by thin\u2010layer chromatography (TLC) and confirmed by liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) and high\u2010performance liquid chromatography (HPLC). Quantification was carried out by HPLC.<\/jats:p><\/jats:sec>Results<\/jats:title>Blue fluorescence indicated a prominent accumulation of CPT in roots compared to leaf with petiole, twigs, and stembark. This accumulation was observed in upper and lower epidermis of the leaf, isolated strands of fibres in the phloem in the petiole, and groups of idioblast cells in the cortex. The ultrasonic\u2010assisted extraction with 90% methanol showed the highest CPT yield in the root (1.91\u2009\u00b1\u20090.02\u2009mg\/g of dry weight), followed by stembark and the least in leaves [0.02\u2009\u00b1\u20090.01\u2009mg\/g (dry weight)] irrespective of the method of extraction. However, hot ethanol extraction gave the highest CPT yield for twig and leaf, indicating the necessity of tissue\u2010specific extraction methods for better recovery of CPT.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3226","type":"journal-article","created":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T21:20:30Z","timestamp":1680643230000},"page":"453-460","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Cellular localisation and quantification of Camptothecin in different plant parts of Nothapodytes nimmoniana<\/i> (J. Graham) Mabberley of Sri Lankan origin"],"prefix":"10.1002","volume":"34","author":[{"given":"Kamalapani Darshani","family":"Degambada","sequence":"first","affiliation":[{"name":"Department of Botany University of Sri Jayewardenepura Nugegoda Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0788-2029","authenticated-orcid":false,"given":"Amila Saman Prasad Kumara","family":"Peramune Arachchilage","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Pharmaceutical Sciences University of Sri Jayewardenepura Nugegoda Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazeera","family":"Salim","sequence":"additional","affiliation":[{"name":"Department of Botany University of Sri Jayewardenepura Nugegoda Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2828-6456","authenticated-orcid":false,"given":"Udumalagala Gamage","family":"Chandrika","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Sri Jayewardenepura Nugegoda Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajita Mahendra","family":"Abeysekera","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Sri Jayewardenepura Nugegoda Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,4]]},"reference":[{"issue":"12","key":"e_1_2_6_2_1","first-page":"2350","article-title":"Camptothecin (CPT) and its derivatives are known to target topoisomerase I (Top1) as their mechanism of action: did we miss something in CPT analogue molecular targets for treating human disease such as cancer?","volume":"7","author":"Li F","year":"2017","journal-title":"Am J Cancer Res"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2004.09.001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221\u20101691(12)60062\u20108"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360\u20101385(00)01575\u20102"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1078\/0176\u20101617\u201000891"},{"issue":"3","key":"e_1_2_6_7_1","first-page":"321","article-title":"Cellular localisation of the anti\u2010cancer drug camptothecin in Camptotheca acuminata Decne (Nyssaceae)","volume":"48","author":"Pasqua G","year":"2004","journal-title":"Eur J Histochem: EJH"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.13189\/azb.2013.010203"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.11.020"},{"issue":"8","key":"e_1_2_6_10_1","first-page":"1142","article-title":"Chemical profiling of Nothapodytes nimmoniana populations in the Western Ghats, India for anti\u2010cancer compound, camptothecin","volume":"92","author":"Suhas S","year":"2007","journal-title":"Curr Sci"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1139\/w05\u2010122"},{"key":"e_1_2_6_12_1","unstructured":"SharmaA NamdeoA MahadikKR Development aaOPAV\u2010I\u2010N.Pharmacognostic Studies On Nothapodytes nimmoniana(J. Graham) Mabberly;2009."},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2021.1946535"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.120.1.43"},{"issue":"5","key":"e_1_2_6_15_1","first-page":"115","article-title":"Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug","volume":"2","author":"Pandey A","year":"2014","journal-title":"J Pharmacogn Phytochem"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1625\/jcam.8.1"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.3226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:58:06Z","timestamp":1761051486000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.3226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,4]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1002\/pca.3226"],"URL":"https:\/\/doi.org\/10.1002\/pca.3226","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"value":"0958-0344","type":"print"},{"value":"1099-1565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,4]]},"assertion":[{"value":"2022-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}