{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:08:04Z","timestamp":1791256084960,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T00:00:00Z","timestamp":1690675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["YDZJ202201ZYTS514"],"award-info":[{"award-number":["YDZJ202201ZYTS514"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Cicer arietinum<\/jats:italic><\/jats:styled-content> L. is the choice of health food for people with diabetes, hypertension, and hyperlipidemia. As an essential source of high\u2010nutrition legumes, it is also an important source of dietary isoflavones.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In order to improve the preparation efficiency of natural plants, a rapid biological activity screening and preparation of xanthine oxidase inhibitors from <jats:styled-content style=\"fixed-case\"><jats:italic>C. arietinum<\/jats:italic><\/jats:styled-content> L. was established.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Xanthine oxidase (XOD) inhibitors were rapidly screened using ultrafiltration liquid chromatography\u2013mass spectrometry (UF\u2010LC\u2013MS) based on receptor\u2013ligand affinity. The change in XOD activity was evaluated by enzymatic reaction kinetics measurement. The potential bioactive compounds were verified through molecular docking. In addition, the biological activity of ligands screened was separated and purified by complex chromatography. The structures of the compounds were identified by nuclear magnetic resonance spectroscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Three active ingredients, namely daidzin, daidzein, calycosin with XOD binding affinities were identified and isolated from the raw plant materials via semi\u2010preparative high\u2010performance liquid chromatography (HPLC), 0\u201360\u2009min, 5\u201350% B and countercurrent chromatography (CCC) (ethyl acetate\/acetic acid\/water [5:0.8:10, v\/v\/v]).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study will help to elucidate the mechanisms of action of natural plants of interest at the molecular level and could also provide more opportunities for the discovery and development of new nutritional value from other natural resources.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3267","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T00:19:10Z","timestamp":1690762750000},"page":"997-1008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In\u2010depth analysis of the xanthine oxidase inhibitors of <scp><i>Cicer arietinum<\/i><\/scp> L.\u2010based receptor\u2013ligand affinity coupled with complex chromatography"],"prefix":"10.1002","volume":"34","author":[{"given":"Wanchao","family":"Hou","sequence":"first","affiliation":[{"name":"Central Laboratory Changchun Normal University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huazhou","family":"Niu","sequence":"additional","affiliation":[{"name":"Central Laboratory Changchun Normal University  Changchun 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