{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:51:37Z","timestamp":1786978297712,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:00:00Z","timestamp":1698364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Natural deep eutectic solvents (NADES) have emerged as interesting extractants to develop botanical ingredients. They are nontoxic and biodegradable, nonflammable, easy to prepare, and able to solubilize a wide range of molecules. However, NADES extracts remain difficult to analyze because the metabolites of interest stay highly diluted in the nonvolatile viscous NADES matrix.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study presents a robust analytical workflow for the chemical profiling of NADES extracts. It is applied to\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Hypericum perforatum<\/jats:italic>\n                      <\/jats:styled-content>\n                      aerial parts extracted with the neutral mixture fructose\/glycerol\/water (3\/1\/1, w\/w\/w), and compared to the chemical profiling of a classical dry methanol extract.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Exploiting polarity differences between metabolites, the\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>H. perforatum<\/jats:italic>\n                      <\/jats:styled-content>\n                      NADES extract was partitioned in a liquid\u2013liquid solvent system to trap the hydrophilic NADES constituents in the lower phase. The upper phase, containing a diversity of secondary metabolites from\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>H. perforatum<\/jats:italic>\n                      <\/jats:styled-content>\n                      , was fractionated by centrifugal partition chromatography. All fractions were chemically investigated using a\n                      <jats:sup>13<\/jats:sup>\n                      C NMR dereplication method which involves hierarchical clustering analysis of the whole NMR dataset, a natural metabolite database for metabolite identification, and 2D NMR analyses for validation. Liquid chromatography\u2013mass spectrometry (LC\u2010MS) analyses were also performed to complete the identification process.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A range of 21 metabolites were unambiguously identified, including glycosylated flavonols, lactones, catechins, phenolic acids, lipids, and simple sugars, and 15 additional minor extract constituents were annotated by LC\u2010MS based on exact mass measurements.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The proposed identification process is rapid and nondestructive and provides good prospects to deeply characterize botanical extracts obtained in nonvolatile and viscous NADES systems.<\/jats:p>\n                  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