{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T22:03:40Z","timestamp":1770069820706,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Olive leaves, abundant by\u2010products of the olive oil industry, are a rich source of oleuropein, an important polyphenol in olive leaves. So far, no published methods have been validated using matrix standards for oleuropein quantification in olive leaves.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The study aimed to develop an HPLC method for oleuropein determination in olive leaves using spiked matrix standards prepared from a blank olive leaf matrix, to validate the method with respect to aqueous standards, and cross\u2010validate the HPLC method with UPLC\u2010MS and UPLC\u2010UV techniques.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>Oleuropein was extracted into methanol and analysed by HPLC with fluorescence detection (FLD; excitation and emission wavelengths 281 and 316\u2009nm, respectively) and by UPLC\u2010MS\u2010UV. For validation, calibration curves of spiked matrix standards (0.4 to 4.8\u2009mg\/g) were analysed by the three methods over several days. Oleuropein was then analysed in French olive varieties.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>For the HPLC\u2010FLD method, repeatability and intermediate precision were less than 5% RSD and linearity was demonstrated by the Fischer test. Differences in results of the spiked placebos by the three methods were non\u2010significant, as confirmed by ANOVA. Extraction recovery was &gt;90%, and there was a strong linear relationship between authentic and spiked matrix standards. The determination of oleuropein in French olive varieties is reported, including analysis in \u201cOlivi\u00e8re\u201d cultivar for the first time, leaves of which contained twice the amount of oleuropein compared with \u201cPicholine\u201d.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Accurate quantification of oleuropein is possible using aqueous standards. Cross\u2010validation indicates that selective analysis can equally be carried out by HPLC or by UPLC\u2010MS techniques.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.3302","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T07:38:40Z","timestamp":1700552320000},"page":"476-482","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development and cross\u2010validation of simple HPLC\u2010fluorescence and UPLC\u2010MS\u2010UV methods for rapid determination of oleuropein in olive leaves"],"prefix":"10.1002","volume":"35","author":[{"given":"Morgane","family":"Carrara","sequence":"first","affiliation":[{"name":"Qualisud Mixed Research Unit, Facult\u00e9 de Sciences Pharmaceutiques Universit\u00e9 de Montpellier  Montpellier 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