{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T19:46:53Z","timestamp":1761162413310,"version":"build-2065373602"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Pomegranate (\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Punica granatum<\/jats:italic>\n                      <\/jats:styled-content>\n                      L.) peels are rich in various bioactive compounds. Characterization of these compounds is crucial for the utilization of peel waste in industrial processing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      The study aimed (1) to establish and compare the metabolic profiles of the peel of seven pomegranate cultivars and (2) to identify bioactive compounds contributing to the larvicidal activity against the third instar larvae of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Culex pipiens<\/jats:italic>\n                      <\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      UPLC\u2010ESI\u2010MS\/MS was utilized to analyze peel methanol extracts of different pomegranate cultivars. The larvicidal activity was determined by calculating the larval mortality among the third instar larvae of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. pipiens<\/jats:italic>\n                      <\/jats:styled-content>\n                      . Multivariate data analysis was conducted to identify the metabolites that exhibited a larvicidal effect.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 24 metabolites, including hydrolyzable tannins, flavonoids, and alkaloids, were tentatively identified in both negative and positive ionization modes. The extract of cultivar \u2018Black\u2019 exhibited the most potent larvicidal effect with LC\n                      <jats:sub>50<\/jats:sub>\n                      values of 185.15, 156.84, and 138.12\u00a0ppm\/mL after 24, 48, and 72\u2009h of treatment, respectively. By applying chemometric techniques, the larvicidal activity could be directly correlated to the bioactive compounds punicalagin, quercetin\u2010\n                      <jats:italic>O<\/jats:italic>\n                      \u2010rhamnoside, quercetin\u2010\n                      <jats:italic>O<\/jats:italic>\n                      \u2010pentoside, and galloyl\u2010HHDP\u2010glucose.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The present study implemented UPLC\u2010ESI\u2010MS\/MS and chemometric techniques as potential tools for metabolomics analysis and differentiation between peels of different pomegranate cultivars. In addition, cultivar \u2018Black\u2019 extract could be a promising natural insecticide against mosquitoes since it is rich in bioactive compounds with larvicidal activity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.3307","type":"journal-article","created":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T20:35:31Z","timestamp":1701376531000},"page":"507-520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti\u2010\n                    <scp>\n                      <i>Culex pipiens<\/i>\n                    <\/scp>\n                    activity of different pomegranate cultivars and determination of their bioactive compounds using LC\u2010MS profiling"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5622-1801","authenticated-orcid":false,"given":"Alaa M.","family":"Salem","sequence":"first","affiliation":[{"name":"Pharmacognosy Department, Faculty of 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