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The search for hybrids which are capable of producing specific metabolites favoring adaptation in new climatic conditions is a challenge in cacao farming.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>We aimed to (1) analyze the metabolic changes in calli of three cacao genotypes during water deficit induced by incubation with polyethylene glycol and (2) assess their response to water deficit stress with regard to somatic embryo differentiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Metabolic profiling was carried out using\n                      <jats:sup>1<\/jats:sup>\n                      H\u2010NMR spectroscopy and multivariate data analysis was applied to crude extracts of calli grown in non\u2010stress or water deficit stress conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Water deficit stress influences the capacity of calli to produce embryos. The SCA12 genotype exhibited the best conversion capacity under severe conditions and was considered as tolerant to drought, followed by the SCA6 genotype (mid\u2010tolerant) and the MA12 genotype (sensitive). Fifty\u2010four metabolites were identified in the three cacao genotypes and discriminant metabolites were identified. Metabolites involved in water stress tolerance such as fructose,\n                      <jats:italic>trans<\/jats:italic>\n                      \u2010aconitic acid, leucine, and hydroxybenzene derivatives were observed in SCA12, the tolerant genotype.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These results demonstrate the utility of\n                      <jats:sup>1<\/jats:sup>\n                      H\u2010NMR metabolomics as an essential tool for the analysis of the drought tolerance characteristics of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>T. cacao<\/jats:italic>\n                      <\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.3323","type":"journal-article","created":{"date-parts":[[2024,1,21]],"date-time":"2024-01-21T18:35:25Z","timestamp":1705862125000},"page":"708-722","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Metabolome profiling of cacao (\n                    <scp>\n                      <i>Theobroma cacao<\/i>\n                    <\/scp>\n                    L.) callus under drought stress conditions induced by polyethylene glycol (PEG) as osmoticant"],"prefix":"10.1002","volume":"35","author":[{"given":"Rodrigue Pouengue","family":"Boutchouang","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Sciences University of Yaound\u00e9 I  Yaound\u00e9 Cameroon"},{"name":"Laboratory of Biochemistry and Plant Physiology, Department of Biological Science, Higher Teachers' Training College University of Yaound\u00e9 I  Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oph\u00e9lie","family":"Fliniaux","sequence":"additional","affiliation":[{"name":"BIOPI\u2010BioEcoAgro UMRT 1158 INRAE Universit\u00e9 de Picardie Jules Verne  Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos Victor Evina","family":"Eyamo","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Plant Physiology, Department of Biological Science, Higher Teachers' Training College University of Yaound\u00e9 I  Yaound\u00e9 Cameroon"},{"name":"Department of Agriculture and Agropastoral, Higher Technical Teacher Training College University of Ebolowa  Ebolowa Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Astride Stephanie Mouafi","family":"Djabou","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Plant Physiology, Department of Biological Science, Higher Teachers' Training College University of Yaound\u00e9 I  Yaound\u00e9 Cameroon"},{"name":"Faculty of Agronomy and Agricultural Science University of Dschang  Dschang Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Xavier","family":"Fontaine","sequence":"additional","affiliation":[{"name":"BIOPI\u2010BioEcoAgro UMRT 1158 INRAE Universit\u00e9 de Picardie Jules Verne  Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Molini\u00e9","sequence":"additional","affiliation":[{"name":"BIOPI\u2010BioEcoAgro UMRT 1158 INRAE Universit\u00e9 de Picardie Jules Verne  Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Mesnard","sequence":"additional","affiliation":[{"name":"BIOPI\u2010BioEcoAgro UMRT 1158 INRAE Universit\u00e9 de Picardie Jules Verne  Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0044-7294","authenticated-orcid":false,"given":"Nicolas","family":"Niemenak","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Plant Physiology, Department of Biological Science, Higher Teachers' Training College University of Yaound\u00e9 I  Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,21]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"International Cocoa Organization.Quarterly bulletin of cocoa statistics.2023. vol. 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