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These species are rich in phenolic acid, flavonoids, and hydrolysable tannin.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aimed to assess the biological activities of\n                      <jats:italic>Nymphaea rubra<\/jats:italic>\n                      flowers (NRF) and leaves (NRL) by identifying and quantifying their polyphenolic compounds using ultra\u2010performance liquid chromatography coupled to quadrupole cyclic ion mobility time\u2010of\u2010flight mass spectrometry (UHPLC\u2010Q\u2010cIM\u2010TOF\u2010MS) and triple quadrupole mass spectrometry (UHPLC\u2010TQ\u2010MS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>NRF and NRL powder was extracted with methanol and fractionated using hexane, ethylacetate, and water. Antioxidant and \u03b1\u2010glucosidase, and tyrosinase enzyme inhibitory activities were evaluated. The polyphenolic components of NRF and NRL were identified and quantified using UHPLC\u2010Q\u2010cIM\u2010TOF\u2010MS and UHPLC\u2010TQ\u2010MS. The method was validated using linearity, precision, accuracy, limit of detection (LOD), and lower limit of quantification (LLOQ).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Bioactive substances and antioxidants were highest in the ethylacetate fraction of flowers and leaves. Principal component analysis showed how solvent and plant components affect\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>N. rubra<\/jats:italic>\n                      <\/jats:styled-content>\n                      's bioactivity and bioactive compound extraction. A total of 67 compounds were identified, and among them 21 significant polyphenols were quantified. Each calibration curve had\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009&gt; 0.998. The LOD and LLOQ varied from 0.007 to 0.09 \u03bcg\/mL and from 0.01 to 0.1 \u03bcg\/mL, respectively. NRF contained a significant amount of gallic acid (10.1 mg\/g), while NRL contained abundant pentagalloylglucose (2.8 mg\/g).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The developed method is simple, rapid, and selective for the identification and quantification of bioactive molecules. These findings provide a scientific basis for\n                      <jats:italic>N. rubra<\/jats:italic>\n                      's well\u2010documented biological effects.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.3329","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T23:25:17Z","timestamp":1706743517000},"page":"799-816","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Biological activities, identification, method development, and validation for analysis of polyphenolic compounds in\n                    <i>Nymphaea rubra<\/i>\n                    flowers and leaves by UHPLC\u2010Q\u2010cIM\u2010TOF\u2010MS and UHPLC\u2010TQ\u2010MS"],"prefix":"10.1002","volume":"35","author":[{"given":"Marufa","family":"Naznin","sequence":"first","affiliation":[{"name":"Department of Chemistry Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafiqul","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Chemistry Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8509-6827","authenticated-orcid":false,"given":"Md Badrul","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, Graduate School Kyungpook National University  Daegu South Korea"},{"name":"Food and Bio\u2010Industry Research Institute, Inner Beauty\/Antiaging Center Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maeng\u2010Joon","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Chemistry Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang\u2010Han","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, Graduate School Kyungpook National University  Daegu South Korea"},{"name":"Food and Bio\u2010Industry Research Institute, Inner Beauty\/Antiaging Center Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunghwan","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry Kyungpook National University  Daegu South Korea"},{"name":"Mass Spectrometry Converging Research Center and Green\u2010Nano Materials Research Center  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/7904349"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201032303\u20107"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12020374"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/5796102"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.202100751"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043396"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.afres.2023.100264"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.5530\/pres.15.2.024"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms130910722"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010\u2010014\u20101192\u20108"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b01838"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf500745y"},{"key":"e_1_2_6_14_1","unstructured":"MagnussonB \u00d6rnemarkU eds.Eurachem Guide: The Fitness for Purpose of Analytical Methods \u2013 A Laboratory Guide to Method Validation and Related Topics. 2nd ed.2014. 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