{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:23:16Z","timestamp":1791242596776,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T00:00:00Z","timestamp":1713225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Skin cancer poses a significant health risk globally, necessitating effective and safe therapeutic interventions. Epigallocatechin\u20103\u2010gallate (EGCG) from green tea and rosmarinic acid (RA) from herbs like rosemary offer promising anticancer properties. Combining these compounds may enhance their effectiveness, prompting the need for a reliable analytical method to quantify them.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Herein, we present the development and validation of a high\u2010performance thin\u2010layer chromatography (HPTLC) method for concurrent quantification of EGCG and RA in lipid\u2010based nanoparticles and biological samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      The method underwent optimisation through design of experiments (DoE), resulting in the establishment of robust chromatographic conditions. The separation process utilised aluminium HPTLC plates coated with silica gel 60 F\n                      <jats:sub>254<\/jats:sub>\n                      as the stationary phase, with the mobile phase comprising ethyl acetate, toluene, formic acid, and methanol in a ratio of 4:4:1:1 v\/v.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The retention factor (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sub>f<\/jats:sub>\n                      ) values obtained were 0.38 for EGCG and 0.61 for RA. The method demonstrated linearity over a range of 100\u2013500\u2009ng\/band for both compounds with excellent correlation coefficients. Limits of detection and quantification were determined, indicating high sensitivity. Precision evaluations revealed relative standard deviation below 2%, ensuring method reproducibility. Recovery assays in lipid\u2010based nanoparticles, plasma, and urine samples demonstrated excellent recoveries (96.2%\u2013102.1%). Forced degradation studies revealed minimal degradation under various stress conditions, with photolytic degradation showing the least impact.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The developed HPTLC method offers a rapid, sensitive, and reliable approach for quantifying EGCG and RA, laying the groundwork for their further investigation as anticancer agents alone and in combination therapies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.3360","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T03:59:37Z","timestamp":1713239977000},"page":"2210-2222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Robust high\u2010performance thin\u2010layer chromatography (HPTLC) method for stability assessment and simultaneous quantification of epigallocatechin\u20103\u2010gallate and rosmarinic acid in lipid\u2010based nanoparticles and biological matrices"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7517-3895","authenticated-orcid":false,"given":"Rahul","family":"Koli","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Quality Assurance, KLE College of Pharmacy KLE Academy of Higher Education and Research  Belagavi Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1266-5603","authenticated-orcid":false,"given":"Vinod S.","family":"Mannur","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Quality Assurance, KLE College of Pharmacy KLE Academy of Higher Education and Research  Belagavi Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya P.","family":"Shetti","sequence":"additional","affiliation":[{"name":"Dr Prabhakar Kore, 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